Dye data acquisition method, dye data acquisition device, and dye data acquisition program

9:

• A dye data acquisition device that processes spectrum data that is a distribution of fluorescence intensity values for each of C (C is an integer of 2 or more) detection wavelengths, for N (N is an integer of 2 or more) target objects, wherein the dye data acquisition device is configured to:
• cluster the N target objects into L (L is an integer of 2 or more and N−1 or less) target object groups based on the intensity values of each target object for each of the C detection wavelengths, and generate L cluster matrices in which the intensity values for each of the C detection wavelengths are arranged for each clustered target object group;
• calculate a statistical value of the intensity values of the target object groups in the C detection wavelengths for each of the L cluster matrices;
• and
• use the statistical values in the C detection wavelengths for each of the L cluster matrices to perform unmixing with respect to the C detection wavelengths, and generate K (K is an integer of 2 or more and C or less) pieces of dye data indicating a distribution for each of K fluorescent dyes.

11:

• A dye data acquisition program for generating dye data indicating a distribution of fluorescent dyes in N (N is an integer of 2 or more) target objects based on spectrum data that is a distribution of fluorescence intensity values for each of C (C is an integer of 2 or more) detection wavelengths, for the N target objects, the dye data acquisition program causing a computer to execute:
• clustering the N target objects into L (L is an integer of 2 or more and N−1 or less) target object groups based on the intensity values of each target object for each of the C detection wavelengths, and generating L cluster matrices in which the intensity values for each of the C detection wavelengths are arranged for each clustered target object group;
• calculating a statistical value of the intensity values of the target object groups in the C detection wavelengths for each of the L cluster matrices;
• and
• using the statistical values in the C detection wavelengths for each of the L cluster matrices to perform unmixing with respect to the C detection wavelengths, and generating K (K is an integer of 2 or more and C or less) pieces of dye data indicating a distribution for each of K fluorescent dyes.

12:

• A dye data acquisition method, comprising:
• acquiring spectrum data that is a distribution of fluorescence intensity values for each of C (C is an integer of 2 or more) detection wavelengths, for N (N is an integer of 2 or more) target objects;
• clustering the C detection wavelengths into M (M is an integer of 2 or more and C−1 or less) detection wavelength groups based on the intensity values, for each of the N target objects, and generating M cluster matrices in which the intensity values for each of the N target objects are arranged for each clustered detection wavelength group;
• calculating a statistical value of the intensity values of the detection wavelength groups in the N target objects for each of the M cluster matrices;
• and
• using the statistical values in the N target objects for each of the M cluster matrices to perform unmixing with respect to the N target objects, and generating K (K is an integer of 2 or more and M or less) pieces of dye data indicating a distribution for each of K fluorescent dyes.

13:

• A dye data acquisition device that processes spectrum data that is a distribution of fluorescence intensity values for each of C (C is an integer of 2 or more) detection wavelengths, for N (N is an integer of 2 or more) target objects, wherein the dye data acquisition device is configured to:
• cluster the C detection wavelengths into M (M is an integer of 2 or more and C−1 or less) detection wavelength groups based on the intensity values, for each of the N target objects, and generate M cluster matrices in which the intensity values for each of the N target objects are arranged for each clustered detection wavelength group;
• calculate a statistical value of the intensity values of the detection wavelength groups in the N target objects for each of the M cluster matrices;
• and
• use the statistical values in the N target objects for each of the M cluster matrices to perform unmixing with respect to the N target objects, and generate K (K is an integer of 2 or more and M or less) pieces of dye data indicating a distribution for each of K fluorescent dyes.

14:

• A dye data acquisition program for generating dye data indicating a distribution of fluorescent dyes in N (N is an integer of 2 or more) target objects based on spectrum data that is a distribution of fluorescence intensity values for each of C (C is an integer of 2 or more) detection wavelengths, for the N target objects, the dye data acquisition program causing a computer to execute:
• clustering the C detection wavelengths into M (M is an integer of 2 or more and C−1 or less) detection wavelength groups based on the intensity values, for each of the N target objects, and generating M cluster matrices in which the intensity values for each of the N target objects are arranged for each clustered detection wavelength group;
• calculating a statistical value of the intensity values of the detection wavelength groups in the N target objects for each of the M cluster matrices;
• and
• using the statistical values in the N target objects for each of the M cluster matrices to perform unmixing with respect to the N target objects, and generating K (K is an integer of 2 or more and M or less) pieces of dye data indicating a distribution for each of K fluorescent dyes.