Methods for identifying herbicidal compounds
AssigneeSyngenta Crop Protection AG
InventorChristian Guy NOBLE
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Abstract
The present invention relates to an in vitro method for determining whether or not a candidate compound is an inhibitor of diaminopelargonic acid aminotransferase (DAPA-AT) said method comprising (i) assaying the candidate compound in an assay mixture comprising (a) the DAPA-AT, (b) an amine donor, (c) pyridoxal 5′-phosphate (PLP) and (d) 7-keto-8-aminopelargoic acid (KAPA), and (ii) detecting inhibition of the DAPA-AT by the candidate compound; characterised in that the amine donor comprises an aliphatic diamine or aliphatic polyamine.
Claims15
An in vitro method for determining whether or not a candidate compound is an inhibitor of diaminopelargonic acid aminotransferase (DAPA-AT) said method comprising (i) assaying the candidate compound in an assay mixture comprising (a) the DAPA-AT, (b) an amine donor, (c) pyridoxal 5′-phosphate (PLP) and (d) 7-keto-8-aminopelargoic acid (KAPA), and (ii) detecting inhibition of the DAPA-AT by the candidate compound;
- characterised in that the amine donor comprises an aliphatic diamine or aliphatic polyamine or a carbamate derivative thereof.
Use of an amine donor which comprises a compound of Formula (I):
- wherein X is NH or CH2;
- R1 is selected from the group consisting of hydrogen, —C(O)OH and —[CH2]pNH2;
- m and n are independently selected from 0, 1, 2, 3 or 4;
- and
- p=1, 2, 3 or 4;
- or a carbamate derivative thereof wherein R1 is —C(O)OH;
- in an in vitro diaminopelargonic acid aminotransferase (DAPA-AT) assay.
Dependencies are inferred from the claim text.