Multi-functional lipid nanoparticles and uses thereof
AssigneeUniversity of Tennessee Research Foundation
InventorsDeidra JH MOUNTAIN, Phillip Connor WEST, Richard FISHER
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Abstract
The present disclosure relates to lipid nanoparticles including a lipid layer, a cell-penetrating peptide conjugated to the lipid layer, a collagen-targeting peptide conjugated to the lipid layer and a nucleic acid associated with a vascular disease or condition. The present disclosure also relates to methods of making and using the described lipid nanoparticles for treating vascular disease.
Claims58
A lipid nanoparticle, comprising:
- a lipid layer, wherein the lipid layer comprises a helper lipid, a PEG lipid, a structural lipid, or a combination thereof;
- a cell-penetrating peptide conjugated to the lipid layer;
- a collagen-targeting peptide conjugated to the lipid layer;
- and
- a nucleic acid associated with a vascular disease or condition.
A lipid nanoparticle, comprising:
- a lipid layer, wherein the lipid layer comprises a helper lipid, a PEG lipid, a structural lipid, or a combination thereof;
- a cell-penetrating peptide conjugated to the lipid layer;
- a collagen-targeting peptide conjugated to the lipid layer;
- and
- a biologically active ingredient that comprises one or more of antibiotics, stimulants, statins, b-receptor blockers, anti-hypertensives, anticoagulants, bronchodilators, corticosteroids, insulin, vaccines, immunosuppressants, interferons, antibodies, proteins, and peptides.
A method of treating vascular disease in a subject in need thereof, comprising:
- administering to the subject a lipid nanoparticle comprising:
- a lipid layer;
- a cell-penetrating peptide conjugated to the lipid layer,
- a collagen-targeting peptide conjugated to the lipid layer, and
- a therapeutically effective ingredient encapsulated in the lipid nanoparticle.
A method of preparing lipid nanoparticles, comprising:
- (a) combining a hydrophobic mixture with an aqueous solution, wherein the hydrophobic mixture comprises:
- one or more lipids, wherein the one or more lipids comprise a helper lipid, a PEG lipid, a structural lipid, or a combination thereof,
- a cell-penetrating peptide, and
- a collagen-targeting peptide, and
- wherein the aqueous solution comprises a cation and a biological active ingredient, wherein the biological active ingredient is associated with a vascular disease or condition
- thereby forming the lipid nanoparticle;
- and
- (b) isolating the lipid nanoparticles.
Dependencies are inferred from the claim text.