Detecting degradation of a polynucleotide by raman spectroscopy
AssigneeThe Research Foundation for The State University of New York
InventorsIgor LEDNEV, Alexander SHEKHTMAN, Lamyaa ALMEHMADI, Sergei REVERDATTO
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Abstract
Provided is a method of detecting degradation of a polynucleotide, including irradiating the polynucleotide with ultraviolet light having a wavelength within a range of from about 200 nm to about 280 nm, and detecting Raman scattering by the polynucleotide. A wavenumber shift of a Raman band or change in peak intensity of a Raman band may constitute detection of polynucleotide degradation. In an example the polynucleotide is in a lipid nanoparticle.
Claims48
A method of detecting degradation of a polynucleotide, comprising
- irradiating the polynucleotide with ultraviolet light having a wavelength within a range of from about 200 nm to about 280 nm, and
- detecting Raman scattering by the polynucleotide.
A method of detecting degradation of a test polynucleotide, comprising
- irradiating a control polynucleotide and the test polynucleotide with ultraviolet light having a wavelength within a range of from about 200 nm to about 280 nm,
- detecting Raman scattering by the control polynucleotide and by the test polynucleotide, and
- detecting a difference between the Raman scattering by the test polynucleotide and by the control polynucleotide.
A method of detecting degradation of an RNA polynucleotide, comprising
- irradiating the polynucleotide with ultraviolet light having a wavelength within a range of from about 260 nm to about 270 nm, and
- detecting Raman scattering by the polynucleotide.
The method of 37, wherein the Raman scattering comprises a wavenumber shift of one or more Raman band of at least 2 cm−1.
A method of detecting degradation of a test polynucleotide, comprising
- irradiating a control polynucleotide and the test polynucleotide with ultraviolet light having a wavelength within a range of from about 260 nm to about 270 nm,
- detecting Raman scattering by the control polynucleotide and by the test polynucleotide, and
- detecting a difference between the Raman scattering by the test polynucleotide and by the control polynucleotide.
Dependencies are inferred from the claim text.