TAF1b and ASTE1 Frameshift Primers

A primer for DNA amplification comprising:

• the sequence defined by any one of SEQ ID NO:
• 24 to 28 or;
• the sequence defined by any one of SEQ ID NO:
• 45, 47, 83 or 100;
• the sequence defined by any one of SEQ ID NO:
• 43, 44, 46, 48 or 49;
• or
• the sequence defined by any one of SEQ ID NO:
• 60 to 62;
• the sequence defined by SEQ ID NO:
• 89;
• and wherein X defines a substitution to any nucleotide which is mismatched to the corresponding nucleotide in the target sequence and the wild type sequence.

A primer for DNA amplification comprising:

• the sequence defined by any one of SEQ ID NO:
• 17 to 19 or 21 to 23;
• the sequence defined by any one of SEQ ID NO:
• 34 to 38 or 40;
• the sequence defined by any one of SEQ ID NO:
• 39, 41, 42, 84-88, 98, 99 or 101;
• the sequence defined by any one of SEQ ID NO:
• 53 to 59;
• or
• the sequence defined by SEQ ID NO:
• 90 or 91.

A method for detecting a mutation in a microsatellite contained in a target sequence of a double-stranded DNA molecule, wherein the mutation is a frameshift of the microsatellite as compared with the corresponding wild type microsatellite sequence, and the method comprises:

• a) providing a first aliquot of a sample comprising human DNA,
• b) adding to the first aliquot the necessary components for DNA amplification and a first primer to form a first reaction mix,
• wherein the first primer is suitable for detection of a mutation in a microsatellite contained in a target sequence of a double stranded DNA molecule and comprises a region of nucleotides that is complementary to the target sequence containing the microsatellite having a frameshift mutation except for between one and four nucleotides which are mismatched to the target sequence containing the microsatellite having a frameshift mutation and which are also mismatched to a corresponding sequence containing the wild type microsatellite, and wherein the first primer is configured to anneal across the length of the microsatellite having a frameshift mutation and to anneal to at least one nucleotide flanking the 3′ end of the microsatellite having a frameshift mutation and at least one nucleotide flanking the 5′ end of the microsatellite having a frameshift mutation,
• c) carrying out DNA amplification on the first reaction mix,
• d) detecting the presence of the frameshift mutation in the sample when an amplification product is produced from the DNA amplification of the first reaction mix.

The method of claim 24, further comprising:

• a) also adding to the first aliquot at step b) of the method either:
• I) the second primer and a third primer, wherein the third primer is configured to anneal 5′ upstream of the region to which the second primer is configured to anneal, and wherein the third primer is configured to anneal to the same strand as the first primer and the opposite strand to the second primer or
• II) a control primer pair, to form the first reaction mix,
• and detecting that DNA amplification has been carried out successfully when an amplification product is produced from the DNA amplification using the second and third primers or the control primer pair;
• or
• b) providing a second aliquot of the sample comprising human DNA and adding to the second aliquot the necessary components for DNA amplification and either:
• I) the second primer and a third primer, wherein the third primer is configured to anneal 5′ upstream of the region to which the second primer is configured to anneal, and wherein the third primer is configured to anneal to the same strand as the first primer and the opposite strand to the second primer or
• II) a control primer pair, to form a second reaction mix, and
• carrying out DNA amplification on the second reaction mix, and detecting that DNA amplification has been carried out successfully when an amplification product is produced from the DNA amplification of the second reaction mix.