Method for Constructing In situ Sequencing Library, and In situ Sequencing Method and Application

Abstract

Provided are a method for constructing an in situ sequencing library, and an in situ sequencing method and an application. The construction method includes: placing a sample on a solid support having an RNA capture function, and then performing non-crosslinking fixation and capturing on mRNA in the tissue sample or cell sample; reverse transcribing the captured mRNA into cDNA, and removing residual tissue in the reverse-transcribed tissue sample or residual cells in the reverse-transcribed cell sample, thereby obtaining a solid support with impurities removed, wherein the cDNA is immobilized on the solid support with impurities removed; and performing target capture and amplification on the cDNA on the solid support with impurities removed, thereby obtaining an amplification product serving as an in situ sequencing library.

Dependencies are inferred from the claim text.