Methods and compositions for production and purification of peptides
AssigneeMicropep Technologies
InventorsOrso SUBRINI, Renaud CARTIER, Mikaêl Stéphane COURBOT
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Abstract
Provided herein are methods of producing peptides from inclusion bodies by linking two or more oligopeptides to an insoluble carrier polypeptide like onconase or TAF12, wherein the oligopeptides are released from the insoluble carrier protein by sequence-specific, chemical cleavage of the peptide bond using acids like acetic acid. Also provided herein are onconase variants with improved properties.
Claims23
-179. (canceled)
A method of producing oligopeptides in a bacteria cell, comprising:
- expressing a fusion polypeptide comprising an insoluble carrier polypeptide operably linked to two or more oligopeptides by a peptide bond, and
- releasing the two or more oligopeptides from the insoluble carrier polypeptide by sequence-specific chemical cleavage of the peptide bond,
- wherein the insoluble carrier polypeptide comprises a TAF12 polypeptide that consists of SEQ ID NO:23, or a modified TAF12 polypeptide defined as having an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% to SEQ ID NO:23.
A fusion polypeptide comprising an insoluble carrier polypeptide operably linked to two or more oligopeptides, wherein the operably linkage between the two or more oligopeptides and the insoluble carrier polypeptide comprise a peptide bond that is capable of sequence-specific chemical cleavage, wherein the insoluble carrier polypeptide comprises a TAF12 polypeptide that consists of SEQ ID NO:23, or a modified TAF12 polypeptide defined as having an amino acid sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% to SEQ ID NO:23.
Dependencies are inferred from the claim text.