Gene encoding cytochrome p450 and use thereof
AssigneeKumiai Chemical Industry Co., Ltd.
InventorsShunsuke YAMAMOTO, Kiyoshi KAWAI
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Abstract
Metabolic activity is exhibited against a larger number of compounds having different acting properties. A cytochrome P450 gene encodes a protein having an amino acid sequence of any of SEQ ID NOS: 2, 4, 6, and 8.
Claims4
A method for controlling weed noxious to a transgenic plant, comprising treating a field used for cultivating the transgenic plant with at least one inhibitor selected from the group consisting of an acetolactate synthase inhibitor classified as code 2 of Herbicide Resistance Action Committee (HRAC) classifications, a microtubule assembly inhibitor classified as code 3 of HRAC classifications, a photosystem II inhibitor classified as code 6 of HRAC classification, a carotenoid biosynthesis inhibitor in phytoene desaturase (PDS) classified as code 12 of HRAC classifications, a protoporphyrinogen oxidase inhibitor classified as code 14 of HRAC classifications, a dihydropteroate synthase inhibitor classified as code 18 of HRAC classifications, and a 4-hydroxyphenylpyruvate dioxygenase inhibitor classified as code 27 of HRAC classifications,
- wherein the transgenic plant comprises an expression vector, and
- wherein the expression vector comprises a cytochrome P450 gene encoding a protein described in any of the following (a) to (c):
- (a) a protein comprising an amino acid sequence of any of SEQ ID NOS:
- 2, 4, 6, and 8;
- (b) a protein comprising an amino acid sequence having 80% or more identity to the amino acid sequence of any of SEQ ID NOS:
- 2, 4, 6, and 8, and having metabolic activity against a microtubule assembly inhibitor classified as code 3 of HRAC classifications, a carotenoid biosynthesis inhibitor in phytoene desaturase (PDS) classified as code 12 of HRAC classifications, a protoporphyrinogen oxidase inhibitor classified as code 14 of HRAC classifications, and a dihydropteroate synthase inhibitor classified as code 18 of HRAC classifications;
- and
- (c) a protein comprising an amino acid sequence encoded by a polynucleotide that hybridizes, under stringent conditions, with a polynucleotide consisting of a nucleotide sequence complementary to a nucleotide sequence of any of SEQ ID NO:
- 1, 3, 5, and 7, and having metabolic activity against a microtubule assembly inhibitor classified as code 3 of HRAC classifications, a carotenoid biosynthesis inhibitor in phytoene desaturase (PDS) classified as code 12 of HRAC classifications, a protoporphyrinogen oxidase inhibitor classified as code 14 of HRAC classifications, and a dihydropteroate synthase inhibitor classified as code 18 of HRAC classifications.
Dependencies are inferred from the claim text.