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US 2026/0218168 A1Published application
Fine needle aspiration methods
AssigneeBioSkryb Genomics, Inc.
InventorJay A.A. WEST
Life of this patent
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Abstract
Provided herein are compositions and methods for high-throughput Primary Template-Directed Amplification (PTA) nucleic acid amplification and sequencing methods, and their applications for mutational analysis in research, diagnostics, and treatment. Further provided herein are methods for obtaining samples using fine needle aspiration.
Claims44
A method of spatial nucleic acid analysis comprising:
- (a) providing a sample comprising a heterogeneous population of cells, wherein each cell has a unique location in the sample, and the sample was obtained using fine needle aspiration;
- (b) isolating one or more cells from the population of cells, wherein the location of the one or more cells is recorded;
- (c) amplifying DNA from one or more cells in the population of cells;
- and
- (d) generating a genotype from the DNA, wherein the location of the one or more cells and the corresponding genotype are preserved.
2 more elements
A method of producing at least one map for visualizing different cell subtypes or cell states in a heterogeneous population of cells comprising:
- (a) providing a sample comprising a heterogeneous population of cells, wherein each cell has a unique location in the sample, and the sample was obtained using fine needle aspiration;
- (b) isolating one or more cells from the population of cells, wherein the location of the one or more cells is recorded;
- (c) amplifying DNA from one or more cells in the population of cells;
- (d) sequencing the amplified DNA from the one or more cells to generate a genotype;
- and
- (e) generating at least one map which correlates the location of the one or more cells with the genotype.
3 more elements
A system for spatial nucleic acid analysis comprising:
- a sample comprising one or more cells, wherein the sample was obtained using fine needle aspiration;
- a device comprising:
- i. a cell collection module;
- ii. an objective for visualizing single cells in the sample;
- and
- iii. a robotic device configured to isolate single cells from the sample;
- and
- at least one reaction chamber for isothermal amplification of nucleic acids from the one or more cells with one or more terminator nucleotides.
5 more elements
Dependencies are inferred from the claim text.