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US 2026/0218140 A1Published application
Engineered polymerase for threose nucleic acid synthesis
AssigneeThe Regents of the University of California
InventorsJohn C. Chaput, Eric J. Yik, Victoria A. Maola
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Abstract
An improved threose nucleic acid (TNA) polymerase exhibits both increased efficiency and increased capability relative to earlier TNA polymerases, with a catalytic rate of ~1 nt/s and >99% fidelity. The new enzyme shows reduced activity against dNTPs, maintains ultrahigh thermal stability, and readily accepts C5-modified TNA nucleoside triphosphates (tNTPs). Also provided are methods for making and using the improved TNA polymerase. The TNA polymerase.
Claims17
A threose nucleic acid (TNA) polymerase comprising an amino acid sequence at least 80% identical to SEQ ID NO:
- 2, wherein the amino acid sequence comprises each of the residues R99, A102, V107, I127, T136, A141, A143, K285, A296, Q297, G304, V337, H339, P340, Y356, R375, Y377, E378, L381, E383, A386, K395, R466, V472, L474, L475, K477, R486, S492, G493, Q520, E523, T524, R527, F533, L538, A540, P548, H550, K562, D566, L575, D602, G607, G615, R672, S717, A724, P741, C749, and T771 of SEQ ID NO:
- 2;
- and wherein the encoded DNA polymerase synthesizes a TNA in the presence of a DNA template and threose nucleotides.
Dependencies are inferred from the claim text.