Novel nkr variants for increased production of isobutanol
AssigneeGevo, Inc.
InventorsChristopher Smith, Timothy Hanly, Jeanne Benoit
Life of this patent
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Abstract
The present invention relates to recombinant microorganisms comprising at least one nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI) or modified NADH-dependent variant thereof, wherein said KARI is at least about 60% identical to SEQ ID NO: 2. In various aspects of the invention, the recombinant microorganisms may comprise an isobutanol producing metabolic pathway and can be used in methods of making isobutanol.
Claims28
An isolated nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI), wherein the KARI is at least about 60% identical to SEQ ID NO:
- 2 and contains at least one substitution modification compared to SEQ ID NO:
- 2.
An isolated nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI), wherein the KARI contains at least one substitution modification compared to SEQ ID NO:
- 2 at positions corresponding to amino acids selected from the group consisting of:
- glycine 31, methionine 77, valine 91, glutamic acid 94, tyrosine 126, glutamine 150, glutamine 165, lysine 290, glutamine 293, glutamine 299, glutamine 330, serine 61, serine 63, and isoleucine 95.
An isolated nucleic acid molecule encoding a ketol-acid reductoisomerase (KARI), wherein the isolated nucleic acid molecule is comprised of SEQ ID NO:
- 89, SEQ ID NO:
- 91, or SEQ ID NO:
- 93.
Dependencies are inferred from the claim text.