Method for regulating degree of cell differentiation
AssigneeNational University Corporation Chiba University
InventorsNaoya TAKAYAMA, Sudip Kumar PAUL, Akihiro NAKAJIMA, YiJing LIU, Sou NAKAMURA
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Abstract
The present invention relates to a method for regulating the degree of cell differentiation including a step of forcibly expressing the MYC family gene and the BMI1 gene in cells selected from the group consisting of (i) mesenchymal stromal cells, vascular endothelial cells, smooth muscle cells, neural crest cells, and lymphocytes having any degree of differentiation, and (ii) megakaryocytes and myelocytes having a high degree of differentiation.
Claims27
A method for regulating the degree of cell differentiation to a lower degree of differentiation, comprising a step of forcibly expressing the MYC family gene and the BMI1 gene in cells selected from the group consisting of (i) mesenchymal stromal cells and lymphocytes having any degree of differentiation induced differentiation from pluripotent stem cells, and (ii) macrophages having a high degree of differentiation induced differentiation from pluripotent stem cells, and obtaining cells having a low degree of differentiation compared to before forced expression.
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A method for producing mesenchymal stromal cells having a proliferative ability, comprising a step of forcibly expressing the MYC family gene and the BMI1 gene in mesenchymal stromal cells having any degree of differentiation induced differentiation from pluripotent stem cells.
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A method for producing lymphocytes having a proliferative ability, comprising a step of forcibly expressing the MYC family gene and the BMI1 gene in lymphocytes having any degree of differentiation induced differentiation from pluripotent stem cells.
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A method for producing macrophages having a proliferative ability, comprising a step of forcibly expressing the MYC family gene and the BMI1 gene in macrophages that express cell surface markers CD14 and CD11b induced differentiation from pluripotent stem cells, and obtaining macrophages in which the expression level of the cell surface marker CD11b decreased compared to before forced expression.
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Dependencies are inferred from the claim text.