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US 2026/0218126 A1Published application
Method for producing retinal tissue
AssigneesRacthera Co., Ltd., Kyoto University
InventorsYasuyuki KITA, Yoriko HORI, Atsushi KUWAHARA, Mototsugu EIRAKU
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Abstract
The present disclosure includes a method of producing a retinal tissue, comprising steps of (1) seeding pluripotent stem cells, (2) culturing the pluripotent stem cells seeded in the step (1), and (3) culturing cells obtained after the step (2); a retinal tissue produced by the method; and a composition comprising the retinal tissue.
Claims9
A method of producing a retinal tissue, comprising steps of
- (1) seeding pluripotent stem cells on a region A of a surface of a culture substrate at a density of 0.5×105 cells/cm2 to 2.5×105 cells/cm2, wherein the culture substrate comprises the region A and a region B on the surface, the region A has cell adhesiveness, and the region B is adjacent to at least a part of the region A and has cell adhesiveness lower than the cell adhesiveness of the region A,
- (2) culturing the pluripotent stem cells seeded in the step (1) in a medium containing a ROCK inhibitor for 1 to 16 hours, and
- (3) culturing cells obtained after the step (2) in a medium containing a BMP signaling activator.
Dependencies are inferred from the claim text.