Systems and methods of preparing and delivering oocyst solutions

A system for disrupting outer enclosures of cells and releasing contents therefrom, the system comprising:

• a first vessel containing cells in suspension, the cells having outer enclosures that contain fluid and microstructures of the cells therein;
• an inline disruption system comprising a vibration source and a cell processing chamber containing vibrating components, the cell processing chamber being configured to receive the suspension from the first vessel and to modify the suspension by disrupting the outer enclosures of at least some of the cells, thereby releasing contents from the disrupted cells and producing a modified solution;
• and
• an outlet connected to the cell processing chamber and configured to deliver the modified solution to a downstream containment vessel or to other system components for additional processing.

A system for disrupting outer membranes of Plasmodium oocysts and releasing viable sporozoites therefrom, the system comprising:

• a vessel containing Plasmodium oocysts in a solution, the Plasmodium oocysts having outer membranes and containing viable sporozoites therein;
• an inline disruption system comprising a vibration source and an oocyst processing chamber containing vibrating rods, the oocyst processing chamber being configured to receive the solution from the vessel and to modify the solution by disrupting the outer membranes of at least some of the Plasmodium oocysts, thereby releasing viable sporozoites from the disrupted Plasmodium oocysts and producing a modified solution;
• and
• an outlet connected to the oocyst processing chamber and configured to deliver the modified solution downstream for further processing.