Amniotic fluid-derived extracellular vesicles and uses thereof for wound healing
AssigneeMerakris Therapeutics LLC
InventorsThomas Christopher Broderick, William Samuel Fagg, IV
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Abstract
The described invention provides compositions and methods for wound healing in a subject. The methods include administering a therapeutic amount of a pharmaceutical composition comprising extracellular vesicles from amniotic fluid and a pharmaceutically acceptable carrier.
Claims17
A method for promoting wound healing in a subject in need thereof comprising contacting a wounded tissue of the subject with a first pharmaceutical composition and a pharmaceutically acceptable carrier comprising a therapeutic amount of a population of isolated extracellular vesicles (EVs) purified from human amnio tic fluid (A), wherein the therapeutic amount is reduces wound area and promotes repair of the wounded tissue, wherein the population of isolated EVs is purified from human AF by:
- (1) providing human AF;
- and
- (2) subjecting the AF to one or more of:
- a. ultracentrifugation;
- b. sucrose density gradient centrifugation;
- c. column chromatography;
- d. size exclusion;
- or
- e. filtration through a device containing an affinity matrix selective towards the EVs;
- and
- (3) obtaining the isolated EVs, thereby purifying the EVs from human AF.
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Dependencies are inferred from the claim text.