Programmable dna proteolysis target chimeras and methods of their use
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsHao YAN, Yang XU, Rong ZHENG, Abhay PRASAD, Deeksha SATYABOLA
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Abstract
Described herein are programmable DNA proteolysis target chimera complexes which can be used to both directly treat a cancer by inhibiting a biochemical pathway overexpressed in a cancer cell and indirectly treat a cancer by recruiting an E3 ligase complex to engage with a protein of interest or mutant thereof and commence proteolysis. Also described herein are methods of using said complexes for treating cancer, and compositions comprising said complexes.
Claims21
A programmable DNA proteolysis target chimera complex comprising:
- a. a first DNA strand comprising one or a plurality of independent E3 ligase ligands;
- and
- b. a second DNA strand comprising one or a plurality of independent protein of interest (POI) targeting ligands,
- wherein at least a portion of the first DNA strand is complementary to a portion of the second DNA strand and the first and second DNA strands form a DNA duplex,
- wherein the one or a plurality of independent E3 ligase ligands is connected to the first DNA strand at one or a plurality of independently selected positions on said first DNA strand, and
- wherein the one or a plurality of independent POI targeting ligands is connected to the second DNA strand at one or a plurality of independently selected positions on said second DNA strand.
The complex of claim of claim 11, further comprising a protein of interest complexed to the one or plurality of POI targeting ligands.
21-29. (canceled)
Dependencies are inferred from the claim text.