Necrosis inhibitor

A method of treating or preventing (i) necrosis, (ii) tissue damage, (iii) organ damage, or (iv) cell death in a subject, the method comprising:

• administering one or more endoplasmic/sarcoplasmic reticulum calcium channel inhibitors and one or more gap junction inhibitors to the subject to treat or prevent necrosis, tissue damage, organ damage, or cell death,
• wherein the one or more endoplasmic/sarcoplasmic reticulum calcium channel inhibitors and the one or more gap junction inhibitors are administered simultaneously, separately or sequentially.

A method of preventing (i) necrosis or (ii) cell death in a cell or tissue, the method comprising:

• contacting the cell or tissue with one or more endoplasmic/sarcoplasmic reticulum calcium channel inhibitors and one or more gap junction inhibitors to prevent necrosis or cell death in the cell or tissue.

23-25. (canceled)

A method of preserving a live tissue or organ, the method comprising culturing or storing the tissue or organ in a culture media comprising one or more endoplasmic/sarcoplasmic reticulum calcium channel inhibitors and one or more gap junction inhibitors to preserve the tissue or organ.

(canceled)

A method of engineering a tissue or an organ, the method comprising contacting the organ or tissue with a culture media comprising one or more endoplasmic/sarcoplasmic reticulum calcium channel inhibitors and one or more gap junction inhibitors.

29-33. (canceled)

A method of preserving or growing cell cultures, the method comprising contacting a culture media with one or more endoplasmic/sarcoplasmic reticulum calcium channel inhibitors and one or more gap junction inhibitors to preserve or grow the cell cultures.

35-42. (canceled)

A combination comprising dantrolene, or a pharmaceutically acceptable salt or a solvate thereof, and quinine, or a pharmaceutically acceptable salt or a solvate thereof.