Method to establish an efficient tissue culture system for rapid aquilaria sinensis propagation

A method to establish an efficient tissue culture system for rapid Aquilaria sinensis propagation, characterize that, comprising the following steps:

• (1) culturing explants of Aquilaria sinensis in an axillary bud induction culture medium, and excising axillary buds from stems to obtain sterile buds;
• and inoculating the sterile buds into a clustered bud induction culture medium for further culture;
• wherein the clustered bud induction culture medium is woody plant medium (WPM)+6-benzylamonipurine (6-BA) 0.1-1 mg/L+1-naphthaleneacetic acid (NAA) 0.1-0.5 mg/L+sucrose 10-30 g/L+Agar 5-10 g/L, pH=5.5-6;
• and
• (2) excising the sterile buds further cultured in the clustered bud induction culture medium in the step (1) from original buds, and transferring the sterile buds to a rooting and seedling strengthening culture medium for further culture until roots begin to develop from bases of the buds;
• the rooting and seedling strengthening culture medium contains ½ Murashige and Skoog (MS) medium+sucrose 20-40 g/L+Agar 5-10 g/L, pH=5.8±1.0.