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US 2026/0210964 A1Published application
METHOD FOR DETECTING DIVIDING CANCER CELLS USING PHOSPHO-Ki67 (Ser 2344) AS MITOTIC BIOMARKER
AssigneeKoc Universitesi
InventorsNazan SANER, Nurhan OZLU SICAKKAN, Ceyda Seren CEYHAN
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Abstract
A use of a biomarker which is for detection of dividing cancer cells based on the mitosis specific phosphorylation of Ki67 at serine 2344 is provided. A reagent (an antibody) against the phosphorylation of Ki67 at serine 2344 and a kit including the reagent are also provided for determining the presence, absence or progression of cancer in a subject.
Claims20
A method for detecting dividing cells in a biological sample in a presence of a mitotic biomarker, comprising the following steps:
- providing the biological sample and preparing a biological extract from the biological sample,
- contacting the biological extract with an antibody that specifically binds to a post-translationally modified biomarker protein in mitosis, wherein the antibody specifically recognizes phosphorylation on a Ki67 protein at serine 2344,
- detecting the presence of the mitotic biomarker in the biological sample by a qualitative or quantitative method,
- thereby detecting a mitotic cancer and normal cells during cell division.
1 more element
A method of diagnosing a cancer in a subject, comprising the following steps:
- isolating a biological extract from a biological sample obtained from the subject,
- measuring a level of phosphorylated Ki67 (Ser 2344),
- comparing the level of phosphorylated Ki67 (Ser 2344) in the biological sample with a level of phosphorylated Ki67 (Ser 2344) in a control sample,
- diagnosising the cancer if the level of phosphorylated Ki67 (Ser 2344) in the biological sample is increased relative to the control sample.
1 more element
Dependencies are inferred from the claim text.