Examination device and examination method

An examination device, comprising:

• a substrate;
• a microneedle that pierces skin to collect a body fluid;
• a body fluid flow path that transfers the body fluid collected by the microneedle;
• and
• an immunoassay structure configured to detect, using an antigen-antibody reaction, a biomarker in the body fluid that has been transferred through the body fluid flow path, wherein
• the microneedle, the body fluid flow path, and the immunoassay unit are disposed on a surface on one side of the substrate,
• the surface on the one side is a surface on a tip end side of the microneedle,
• the body fluid flow path extends in a direction perpendicular to a thickness direction of the substrate on the surface on the one side of the substrate,
• a height of the microneedle is 800 μm or more and 1 mm or less, and
• fine unevenness is formed on a surface of the microneedle and a surface of the body fluid flow path in a vicinity of the microneedle.