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US 2026/0202398 A1Published application
Polypeptide-functionalizing reagents and methods of use
AssigneeQuantum-Si Incorporated
InventorsMathivanan Chinnaraj, John Silvio Vieceli
Life of this patent
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Abstract
Provided herein are compositions and methods for functionalizing a polypeptide for single-molecule analysis. In some aspects, the disclosure provides compositions and methods for immobilizing a peptide barcode to a surface. In some aspects, the disclosure provides compositions and methods for luminescently labeling a protein of interest.
Claims26
A composition comprising:
- an avidin protein comprising at least two biotin binding sites;
- a first biotin moiety bound to at least one biotin binding site on the avidin protein;
- and
- a polyglycine peptide attached to the first biotin moiety through a linker,
- wherein the linker comprises a polyethylene glycol (PEG) moiety.
2 more elements
(canceled)
(canceled)
(canceled)
22-39. (canceled)
41-55. (canceled)
A fusion polypeptide, comprising a protein of interest, an affinity tag, a linker, a cleavage site, a barcode, and a sortase tag.
57-93. (canceled)
Dependencies are inferred from the claim text.