Chromatographic materials including crosslinked streptavidin and biotin and methods of use thereof

Abstract

Disclosed herein are chromatographic materials comprising crosslinked streptavidin particles. The crosslinked streptavidin particles are bound to a biotinylated affinity agent and free biotin. The combined impact of streptavidin crosslinking and binding free biotin to accessible binding sites in streptavidin drastically reduce leaching of streptavidin from the chromatographic materials. The chromatographic materials disclosed herein may be used to prepare affinity chromatographic columns.

Dependencies are inferred from the claim text.