Multiple-flowering cucurbita pepo subsp. pepo plants and methods for their production
AssigneeState of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization
InventorsAmit GUR, Harry Stuart PARIS, Galil TZURI
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Abstract
A Cucurbita pepo subsp. pepo plant is provided. The Cucurbita pepo subsp. pepo plant comprises a genome having a loss of function mutation in a Cp4.1LG13g07780 gene or ortholog thereof, wherein the loss of function mutation confers a multiflowering (mf) trait. Also provided are methods of producing and using such plants.
Claims20
A Cucurbita pepo subsp. pepo plant comprising a genome having a loss of function mutation in a Cp4.1LG13g07780 gene or ortholog thereof, wherein the loss of function mutation confers a multiflowering (mf) trait and wherein when the loss of function mutation is in an introgression from a Cucurbita pepo subsp. ovifera var. Crookneck ‘Supersett’, said introgression is smaller than 700 kb or wherein the plant is not of a variety, seeds of which, having been deposited at NCIMB Ltd. under Accession No. NCIMB 41744 (herein referred to as Multizuq) or progeny thereof or Accession No. NCIMB 41794 (herein referred to as Nizzan) or progeny thereof.
A method of producing a Cucurbita pepo subsp. pepo plant having a multiflowering (mf) trait, the method comprising down-regulating a Cp4.1LG13g07780 gene or ortholog thereof in the Cucurbita pepo subsp. pepo plant to thereby produce a Cucurbita pepo subsp. pepo plant having the mf trait, and wherein when said down-regulating is by crossing with a Cucurbita pepo subsp. ovifera:
- an introgression resultant of said crossing is not that present in seeds having been deposited at NCIMB Ltd. under Accession No. NCIMB 41744 (herein referred to as Multizuq) or progeny thereof or Accession No. NCIMB 41794 (herein referred to as Nizzan) or progeny thereof;
- or
- an introgression resultant of said crossing which comprises said ortholog of Cp4.1LG13g07780 is smaller than 700 kb.
A method of identifying a Cucurbita pepo subsp. pepo plant useful for crossing, the method comprising identifying in a genome of the plant at least one nucleic acid sequence alteration indicative of a multiflowering (mf) trait located between MF1 and MF2 or MF21 and MF26 of chromosome 13 using marker assisted selection (MAS), wherein identification of said at least one nucleic acid sequence variation is indicative of Cucurbita pepo subsp. pepo plant useful for crossing.
Dependencies are inferred from the claim text.