Sidewinder three-way junction dna assembly

A composition, comprising:

• n fragments, wherein n is an integer greater than 2,
• wherein each fragment comprises a first barcode, a first toehold, a second barcode, and a second toehold,
• wherein each fragment comprises a first polynucleotide strand and a second polynucleotide strand,
• wherein the first polynucleotide strand comprises a 5′ overhang and a 3′ overhang,
• wherein the 5′ overhang of the first polynucleotide strand comprises the first barcode, and
• wherein the 3′ overhang of the first polynucleotide strand comprises the second barcode,
• wherein for each (i)th fragment, wherein 1<i<n:
• the first barcode of the (i)th fragment is complementary to the second barcode of the (i−1)th fragment;
• the first toehold of the (i)th fragment is complementary to the second toehold of the (i+1)th fragment;
• the second barcode of the (i)th fragment is complementary to the first barcode of the (i+1)th fragment;
• and
• the second toehold of the (i)th fragment is complementary to the first toehold of the (i−1)th fragment;
• wherein the first barcode of the first fragment is complementary to the second barcode of the (n)th fragment, and
• wherein the second toehold of the first fragment is complementary to the first toehold of the (n)th fragment.