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US 2026/0193662 A1Published application
Proteins with minimal n-terminal initiator methionine
AssigneeUnichem Laboratories Ltd
InventorsDhananjay Sathe, Saravanakumar Iyappan
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Abstract
The present invention relates to a recombinant plasmid, methods and compositions for the expression of recombinant proteins with minimal N-terminal initiator methionine. The present invention also provides strategies for efficient removal of N-terminal initiator methionine in recombinant proteins expressed at industrial scale and provides method for producing proteins with minimal N-terminal initiator methionine.
Claims16
A recombinant plasmid comprising of:
- a nucleotide sequence encoding Methionine Aminopeptidase (MAP) protein or a variant thereof, operably linked to a promoter sequence of SEQ ID NO 5;
- and
- a nucleotide sequence encoding protein of interest operably linked to a promoter sequence of SEQ ID NO 7;
- wherein the recombinant plasmid effects the protein of interest consisting of less than 2% N-terminal initiator methionine.
1 more element
A recombinant plasmid of SEQ ID NO:
- 18, wherein the plasmid further comprises of a nucleotide sequence encoding protein of interest operably linked to a promoter sequence of SEQ ID NO:
- 7.
Dependencies are inferred from the claim text.