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AssigneeAgroSpheres, Inc.
InventorsAmeer Hamza SHAKEEL, Zachery George DAVIS, Joseph Thomas FRANK, Sepehr ZOMORODI, Payam POURTAHERI
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The present disclosure relates to compositions of immobilized enzymes on the surface of achromosomal and/or anucleate cells and uses thereof. In particular, the present disclosure provides genetically engineered minicells with enzymes self-assembled on their surface. The immobilized enzymes on the surface of achromosomal and/or anucleate minicells, has agricultural, industrial, and environmental applications due to their improved stability durability and, reusability. Also, provided are methods for producing and purifying enzyme-immobilized minicells.
An industrially suitable anucleated cell-based enzyme immobilization and delivery platform, comprising:
The anucleated cell-based enzyme immobilization and delivery platform according to 51, wherein the at least two expressed self-assembled enzymes have a complimentary function.
The anucleated cell-based enzyme immobilization and delivery platform according to 51, wherein the at least two expressed self-assembled enzymes act synergistically.
The anucleated cell-based enzyme immobilization and delivery platform according to 51, wherein the at least two expressed self-assembled enzymes each work to carry out a portion of an overall enzymatic reaction.
Dependencies are inferred from the claim text.