Methods of reducing conidiation in cell culture
AssigneesBASF SE, The Regents of the University of California
InventorsStefan Haefner, Katja Gemperlein, Louise N. Glass, Yang Li, Lori Huberman, Florian Drescher
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Abstract
Provided herein is a method of reducing conidiation during cell culture of a T. thermophilus strain, the method comprising growing, in a submerged cell culture, a variant T. thermophilus strain that has reduced res-1 activity as compared to a wild-type T. thermophilus strain.
Claims15
A method of reducing conidiation during cell culture of a T. thermophilus strain, the method comprising growing, in a submerged cell culture, a T. thermophilus strain that has reduced res-1 activity as compared to a wild-type T. thermophilus strain.
A method of increasing production of a heterologous protein in a T. thermophilus strain, the method comprising growing a T. thermophilus strain comprising a nucleic acid encoding a heterologous protein in a submerged culture, wherein the T. thermophilus strain has reduced res-1 activity, wherein the expression level of the heterologous protein produced by the T. thermophilus strain that has reduced res-1 activity is higher than an expression level of a recombinant protein produced by a T. thermophilus strain having wild type res1 activity.
Dependencies are inferred from the claim text.