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US 2026/0191247 A1Published application
Compositions and methods for enhanced expression of heterologous polypeptides
AssigneeBond Pet Foods, Inc.
InventorsKarin Pernilla TURNER AUDIBERT, José Ginés GARCÍA-CERDÁN, Annabel ALONSO, Luis N. BRANDAO, Karl Paul GERHARDT, Patrick John WESTFALL, Raul REVELES
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Abstract
Disclosed are methods and compositions for enhanced expression of a heterologous polypeptide of interest in an organism. The methods generally comprise first transforming the organism with a genetic construct having a heterologous polypeptide of interest and amplifying expression of the polypeptide through in vivo gene amplification.
Claims20
A food ingredient composition comprising:
- a recombinant fungi, wherein the genome of the recombinant fungi comprises at least 2 copies of a tandem amplification region integrated into a locus of a haploinsufficient gene tied to fungi fitness, wherein the tandem amplification region comprises:
- a coding sequence (CDS) of one or more heterologous animal proteins in operable linkage with a first promoter and a terminator, wherein the one or more heterologous animal proteins:
- have less than 25% amino acid sequence identity to proteins endogenous to said fungi;
- and
- are characterized by a molecular weight between 5 kDa and 80 kDa;
- at least 5% of the one or more heterologous animal protein by dry cell weight;
- and
- at least 50% total protein by dry cell weight.
5 more elements
Dependencies are inferred from the claim text.