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US 2026/0183345 A1Published application
Methods of electrospray drying anaerobic bacteria and compositions thereof
AssigneeAxiota U.S., Inc.
InventorsMing Woei LAU, James Scott DROUILLARD, Amanda PETERSON, Celine Caroline APERCE, Gina Rae HERREN
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Abstract
The present disclosure relates to methods and systems of electrospray drying anaerobic bacterial cells and/or Megasphaera elsdenii, compositions and feed additives thereof. The methods, compositions, and feed additives thereof will result in improved viability and yield after extended storage of the bacteria. Such improvements are beneficial for a range of applications, including uses in animal health and feeds.
Claims92
A system for spray drying Megasphaera elsdenii cells into a dried powder, the system comprising:
- a source of water, a carrier, and Megasphaera elsdenii cells;
- a tank comprising a stirring device and the tank is arranged to receive the water, the carrier, and the Megasphaera elsdenii cells from the source to form a slurry having a viscosity in the range of about 1 cP to about 500 cP;
- an electrode configured to apply an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- an atomizer;
- a drying chamber comprising an inlet end, an outlet end, and an internal volume located between the inlet end and the outlet end, the internal volume being configured to hold the slurry and a drying fluid,
- wherein the drying chamber is configured to dry the slurry;
- wherein the atomizer is configured to:
- (i) receive the slurry from the tank;
- and
- (ii) discharge the slurry into the drying chamber for contact with the drying fluid to form the dried powder containing the Megasphaera elsdenii cells;
- and
- wherein the drying fluid comprises nitrogen or argon at a temperature between about 50° C. to about 100° C., and wherein the entire system is under less than 2% oxygen.
9 more elements
A method of electrospray drying Megasphaera elsdenii cells into a dried powder, comprising:
- preparing a culture comprising Megasphaera elsdenii cells, a growth media, and an osmolyte protectant molecule;
- harvesting the Megasphaera elsdenii cells;
- forming a slurry comprising water, a carrier, and the Megasphaera elsdenii cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the Megasphaera elsdenii cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
5 more elements
A method of electrospray drying Megasphaera elsdenii cells into a dried powder, comprising:
- preparing a culture comprising Megasphaera elsdenii cells, a growth media, and an osmolyte protectant molecule, wherein during the culture the pH, temperature, and/or osmolality are altered for a time period;
- harvesting the Megasphaera elsdenii cells;
- forming a slurry comprising water, a carrier, and the Megasphaera elsdenii cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the Megasphaera elsdenii cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
5 more elements
A method of electrospray drying Megasphaera elsdenii cells into a dried powder, comprising:
- preparing a culture comprising Megasphaera elsdenii cells, and a growth media;
- adding an osmolyte protectant molecule;
- harvesting the Megasphaera elsdenii cells;
- forming a slurry comprising water, a carrier, and the Megasphaera elsdenii cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the Megasphaera elsdenii cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
6 more elements
A method of electrospray drying Megasphaera elsdenii cells into a dried powder, comprising:
- preparing a culture comprising Megasphaera elsdenii cells, and a growth media, wherein during the culture the pH, temperature, and/or osmolality are altered for a time period;
- adding an osmolyte protectant molecule;
- harvesting the Megasphaera elsdenii cells;
- forming a slurry comprising water, a carrier, and the Megasphaera elsdenii cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the Megasphaera elsdenii cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
6 more elements
A method of electrospray drying Megasphaera elsdenii cells into a dried powder, comprising:
- preparing a culture comprising Megasphaera elsdenii cells, and a growth media;
- harvesting the Megasphaera elsdenii cells;
- adding an osmolyte protectant molecule;
- forming a slurry comprising water, a carrier, and the Megasphaera elsdenii cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the Megasphaera elsdenii cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
6 more elements
A method of electrospray drying Megasphaera elsdenii cells into a dried powder, comprising:
- preparing a culture comprising Megasphaera elsdenii cells, and a growth media, wherein during the culture the pH, temperature, and/or osmolality are altered for a time period;
- harvesting the Megasphaera elsdenii cells;
- adding an osmolyte protectant molecule;
- forming a slurry comprising water, a carrier, and the Megasphaera elsdenii cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the Megasphaera elsdenii cells encapsulated within the carrier, wherein the dried powder comprises less than 10% moisture content, and wherein the entire method is performed under less than 2% oxygen.
6 more elements
A method of forming a dry powder comprising Megasphaera elsdenii cells encapsulated within a carrier, said method comprising:
- forming a slurry in a mixing tank comprising water, a carrier, and Megasphaera elsdenii cells while stirring the mixing tank with the stirring device;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry in an atomizer and discharging atomized slurry into a drying chamber for contact with a drying fluid to form the dried powder containing the Megasphaera elsdenii cells encapsulated within the carrier, and wherein the drying fluid comprises nitrogen or argon at a temperature between about 50° C. to about 100° C., and wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
A system for spray drying anaerobic cells into a dried powder, the system comprising:
- a source of water, a carrier, and anaerobic cells;
- a tank comprising a stirring device and the tank is arranged to receive the water, the carrier, and the anaerobic cells from the source to form a slurry having a viscosity in the range of about 1 cP to about 500 cP;
- an electrode configured to apply an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- an atomizer;
- a drying chamber comprising an inlet end, an outlet end, and an internal volume located between the inlet end and the outlet end, the internal volume being configured to hold the slurry and a drying fluid,
- wherein the drying chamber is configured to dry the slurry;
- wherein the atomizer is configured to:
- (i) receive the slurry from the tank;
- and
- (ii) discharge the slurry into the drying chamber for contact with the drying fluid to form the dried powder containing the anaerobic cells;
- and
- wherein the drying fluid comprises nitrogen or argon at a temperature between about 50° C. to about 100° C., and wherein the entire system is under less than 2% oxygen.
9 more elements
A method of electrospray drying anaerobic cells into a dried powder, comprising:
- preparing a culture comprising anaerobic cells, a growth media, and an osmolyte protectant molecule;
- harvesting the anaerobic cells;
- forming a slurry comprising water, a carrier, and the anaerobic cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the anaerobic cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
5 more elements
A method of electrospray drying anaerobic cells into a dried powder, comprising:
- preparing a culture comprising anaerobic cells, a growth media, and an osmolyte protectant molecule, wherein during the culture the pH, temperature, and/or osmolality are altered for a time period;
- harvesting the anaerobic cells;
- forming a slurry comprising water, a carrier, and the anaerobic cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 10° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the anaerobic cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
5 more elements
A method of electrospray drying anaerobic cells into a dried powder, comprising:
- preparing a culture comprising anaerobic cells, and a growth media;
- adding an osmolyte protectant molecule;
- harvesting the anaerobic cells;
- forming a slurry comprising water, a carrier, and the anaerobic cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the anaerobic cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
6 more elements
A method of electrospray drying anaerobic cells into a dried powder, comprising:
- preparing a culture comprising anaerobic cells, and a growth media, wherein during the culture the pH, temperature, and/or osmolality are altered for a time period;
- adding an osmolyte protectant molecule;
- harvesting the anaerobic cells;
- forming a slurry comprising water, a carrier, and the anaerobic cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the anaerobic cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
6 more elements
A method of electrospray drying anaerobic cells into a dried powder, comprising:
- preparing a culture comprising anaerobic cells, and a growth media;
- harvesting the anaerobic cells;
- adding an osmolyte protectant molecule;
- forming a slurry comprising water, a carrier, and the anaerobic cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the anaerobic cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
6 more elements
A method of electrospray drying anaerobic cells into a dried powder, comprising:
- preparing a culture comprising anaerobic cells, and a growth media, wherein during the culture the pH, temperature, and/or osmolality are altered for a time period;
- harvesting the anaerobic cells;
- adding an osmolyte protectant molecule;
- forming a slurry comprising water, a carrier, and the anaerobic cells, wherein the carrier has a final concentration of about 1% to about 50% wt and the slurry has a viscosity in the range of about 1 cP to about 500 cP;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry to generate a spray of liquid droplets of the slurry;
- introducing the spray of liquid droplets of the slurry into a drying chamber;
- and
- feeding a drying fluid at a temperature between about 50° C. to about 100° C. into the drying chamber to dry the liquid droplets to form the dried powder comprising a plurality of dried particles containing the anaerobic cells encapsulated within the carrier, wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
6 more elements
A method of forming a dry powder comprising anaerobic cells encapsulated within a carrier, said method comprising:
- forming a slurry in a mixing tank comprising water, a carrier, and anaerobic cells while stirring the mixing tank with the stirring device;
- applying an electrostatic charge to the slurry using a voltage gradient or oscillating voltages;
- atomizing the slurry in an atomizer and discharging atomized slurry into a drying chamber for contact with a drying fluid to form the dried powder containing the anaerobic cells encapsulated within the carrier, and wherein the drying fluid comprises nitrogen or argon at a temperature between about 50° C. to about 100° C., and wherein the dried powder comprises less than 15% moisture content, and wherein the entire method is performed under less than 2% oxygen.
Dependencies are inferred from the claim text.