Gene delivery nanoparticle, and preparation and application thereof

A method for preparing a gene delivery nanoparticle, comprising:

• subjecting 2-((tert-butoxycarbonyl)amino)ethyl methacrylate as a monomer, methoxy poly(ethyleneglycol)5k-reversible addition-fragmentation chain transfer agent (mPEG5k-RAFT) as a chain transfer agent and azobisisobutyronitrile as an initiator to a reversible addition-fragmentation chain-transfer polymerization reaction in dimethylformamide, followed by dialysis and freeze-drying to obtain an intermediate;
• dissolving the intermediate in dichloromethane, followed by addition of trifluoroacetic acid and reaction to obtain polyethylene glycol-poly(aminoethyl methacrylate);
• dissolving sparfloxacin in a hydrochloric acid solution, followed by addition of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide to produce a first solution;
• dissolving the polyethylene glycol-poly(aminoethyl methacrylate) in dimethyl sulfoxide to produce a second solution;
• adding the second solution to the first solution to produce a mixture;
• and subjecting the mixture to dialysis and freeze-drying to obtain a final product polyethylene glycol-poly(sparfloxacin);
• and
• placing the polyethylene glycol-poly(sparfloxacin) in an aqueous solution for self-assembly to form the gene delivery nanoparticle.