Host cell with increased cellular reducing power from a heterologous hydrogenase
AssigneeThe Regents of the University of California
InventorsJustin PANICH, Robert BERTRAND, Steven W. SINGER, Jay D. KEASLING
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Abstract
The present invention provides a genetically modified host cell comprising a heterologous hydrogenase, or dehydrogenase, or homologous variant thereof, wherein the genetically modified host cell has an increased cellular reducing power compared an unmodified host cell. In some embodiments, the hydrogenase is a soluble hydrogenase (SH) from Cupriavidus necator H16, or a homologous variant thereof.
Claims20
A system for introducing a nucleic acid encoding a hydrogenase, and/or a dehydrogenase, into a host cell comprising:
- (a) an expression vector comprising hydrogenase genes, and/or dehydrogenase genes, operatively linked to a promoter;
- and (b) a delivery vector encoding genes to integrate the hydrogenase genes, and/or dehydrogenase genes, and the promoter into a chromosome of the host cell.
A genetically modified host cell comprising a nucleic acid comprising:
- (a) one or more nucleotide sequence(s) encoding a hydrogenase, dehydrogenase, or homologous thereof, each independently operatively linked to a promoter;
- and (b) optionally a further nucleotide sequence encoding a NAD(P) transhydrogenase operatively linked to a promoter;
- and (c) optionally a further nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter, wherein the genetically modified host cell has an increased cellular reducing power compared an unmodified host cell.
A method for introducing a nucleic acid encoding a heterologous hydrogenase into a host cell comprising:
- (a) introducing a nucleic acid comprising:
- (i) one or more nucleotide sequence(s) encoding a hydrogenase, dehydrogenase, or homologous thereof, each independently operatively linked to a promoter;
- and (ii) optionally a further nucleotide sequence encoding a NAD(P) transhydrogenase operatively linked to a promoter;
- and (iii) optionally a further nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter, into a host cell, and (b) optionally integrating the nucleic acid into a chromosome of the host cell.
Dependencies are inferred from the claim text.