Evaluation Method for Potential Toxic Effect of Apolygus Lucorum Using In Vitro Tissue from Indoor Living Plant

An evaluation method for a potential toxic effect of Apolygus lucorum using an indoor living plant, comprising the following steps:

• determining a toxic effect of a chemical pesticide on the Apolygus lucorum and/or evaluating an effect of an insect-resistant transgenic plant on a non-target organism comprising the Apolygus lucorum in a laboratory;
• wherein
• determining the toxic effect of the chemical pesticide on the Apolygus lucorum in the laboratory comprises:
• spraying the chemical pesticide on an edible plant of the Apolygus lucorum to obtain a treatment group, taking the edible plant not sprayed with the chemical pesticide as a control group, collecting plant tissues from the treatment group and the control group and placing in an artificial insect-raising container, inoculating nymphs of the Apolygus lucorum as test insects on the plant tissues to allow a test, observing and recording growth and development of the test insects, and analyzing the toxic effect of the chemical pesticide on the Apolygus lucorum according to research purpose and requirement of the test;
• if the chemical pesticide does not produce an acute toxicity, observing a chronic toxicity of the chemical pesticide;
• if there is no significant difference in a development progress of the test insects in the treatment group and the control group, terminating the test 24 h after all the test insects in the control group have emerged;
• if there are a few test insects in the control group that have significantly slow growth and development, terminating the test when an emergence rate of the test insects in the control group reaches not less than 85% while remaining surviving test insects have not reached an age of 5;
• and
• evaluating the effect of the insect-resistant transgenic plant on the non-target organism comprising the Apolygus lucorum in the laboratory comprises:
• taking the insect-resistant transgenic plant not sprayed with the chemical pesticide as a treatment group, taking a non-transgenic plant not sprayed with the chemical pesticide as the control group, taking the non-transgenic plant sprayed with the chemical pesticide as a positive control group, collecting plant tissues from the treatment group, the control group, and the positive control group and placing in the artificial insect-raising container, inoculating the nymphs of the Apolygus lucorum as test insects on the plant tissues to allow a test, and observing and recording growth and development of the test insects;
• if there is no significant difference in a development progress of the test insects in the treatment group and the control group, terminating the test 24 h after all the test insects in the control group have emerged;
• if there are a few test insects in the control group that have significantly slow growth and development, terminating the test when an emergence rate of the test insects in the control group reaches not less than 85% while remaining surviving test insects have not reached an age of 5.