Method for preparing sample containing tumor cells circulating in blood

A method for analyzing circulating tumor cells derived from a subject without nucleic acid amplification and/or proliferation by culture, the method comprising the steps of:

• isolating monocytes from peripheral blood mononuclear cells collected from the subject using apheresis, wherein apheresis condition for performing the apheresis includes circulating tumor cell enrichment condition;
• isolating circulating tumor cells from the peripheral blood mononuclear cells from which the monocytes have been isolated;
• and
• performing analysis using the circulating tumor cells.

The method according to claim 24, further comprising the steps of:

• creating a DNA/RNA sequence library from the DNA or RNA;
• and
• identifying a neoantigen based on mutation information in the sequence library,
• wherein the circulating tumor cells have a purity of at least about 20%.

A method for producing a cell for dendritic cell therapy using a neoantigen possessed by a subject, the method comprising:

• isolating monocytes from peripheral blood mononuclear cells collected from the subject using apheresis, wherein apheresis condition for performing the apheresis includes circulating tumor cell enrichment condition;
• isolating circulating tumor cells from the peripheral blood mononuclear cells from which the monocytes have been isolated;
• extracting DNA or RNA from the circulating tumor cell to create a DNA/RNA sequence library;
• identifying a neoantigen based on mutation information of the sequence library;
• and
• introducing the neoantigen into dendritic cells.

A method for treating or preventing a subject by analyzing circulating tumor cells without nucleic acid amplification and/or proliferation by culture, the method comprising the steps of:

• isolating monocytes from peripheral blood mononuclear cells collected from the subject using apheresis, wherein apheresis condition for performing the apheresis includes circulating tumor cell enrichment condition;
• isolating circulating tumor cells from the peripheral blood mononuclear cells from which the monocytes have been isolated;
• performing analysis using the circulating tumor cells;
• and
• treating or preventing the subject based on results of the analysis.