Methods and composition for novel diterpene synthesis inhibitor herbicide resistant rice lines
AssigneeRiceTec, Inc.
InventorsDario Bernacchi, Caleb Knepper, Anuj Kumar
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Abstract
Rice is described that is tolerant/resistant to high-dose applications of the diterpene biosynthesis inhibiting herbicide clomazone. Putative causal non-target site mutations were identified on chromosome 2 of rice. Use of the high clomazone dose tolerant rice lines for more effective weed control, as well as methods of producing tolerant/resistant rice are also disclosed.
Claims20
A monocot plant comprising a first mutation at chromosome 2 position 16835660 and/or a second mutation at chromosome 2 position 17087862,
- wherein the first mutation comprises a G to T single nucleotide mutation in cDNA position 595 resulting in amino acid change from aspartic acid to tyrosine at expressed protein position 199,
- wherein the second mutation comprises a G to T single nucleotide mutation in cDNA position 903 resulting in amino acid change from glutamine to histidine at expressed protein position 301, and
- wherein the monocot plant is tolerant/resistant to diterpene inhibitors, at levels higher than those tolerated by plants without either of the mutations.
A method for controlling weeds in a rice field, the method comprising:
- a) having a rice plant in the rice field wherein the rice is tolerant/resistant to one or more diterpene inhibiting herbicide;
- and
- b) contacting the rice field with at least one of the diterpene inhibiting herbicide to which the rice is resistant at levels known to induce visible symptoms of phytotoxicity in rice plants,
- wherein the tolerance/resistance is associated with a mutation in genome of the rice at chromosome 2 position 16835660 comprising a G by T single nucleotide mutation in cDNA position 595 resulting in amino acid change from aspartic acid to tyrosine at expressed protein position 199, and/or a mutation at chromosome 2 position 17087862 comprising a G by T single nucleotide mutation in cDNA position 903 resulting in amino acid change from glutamine to histidine at expressed protein position 301, and
- wherein the visible symptoms of phytotoxicity comprise bleaching of leaf tissue, reduced growth rate, reduced plant height, delayed emergence, plant injury, or plant death.
A method of producing rice resistant to diterpene inhibitors, at levels significantly higher than those tolerated by plants without genetic regions conferring tolerance/resistance, the method comprising:
- a) obtaining genetic regions that are equivalents of regions in genome of rice designated that confer tolerance/resistance;
- and
- b) introducing the genetic regions into rice lines to replace wild type regions,
- wherein the tolerance/resistance is associated with a mutation at chromosome 2 position 16835660 comprising a G by T single nucleotide mutation in cDNA position 595 resulting in amino acid change from aspartic acid to tyrosine at expressed protein position 199, and/or a mutation at chromosome 2 position 17087862 comprising a G by T single nucleotide mutation in cDNA position 903 resulting in amino acid change from glutamine to histidine at expressed protein position 301.
A rice seed wherein a representative sample of seed was deposited under ATCC Accession No. PTA-127860.
A method of producing a herbicide resistant rice plant;
- the method comprising modifying genome of a rice plant,
- wherein the modifying comprises introducing a mutation at chromosome 2 position 16835660 comprising a G by T single nucleotide mutation in cDNA position 595 resulting in amino acid change from aspartic acid to tyrosine at expressed protein position 199, and/or a mutation at chromosome 2 position 17087862 comprising a G by T single nucleotide mutation in cDNA position 903 resulting in amino acid change from glutamine to histidine at expressed protein position 301, and
- wherein the modifying confers resistance to an herbicide selected from the group consisting of clomazone, bixlozone, or a combination thereof.
Dependencies are inferred from the claim text.