Devices for storage and delivery of nonpathogenic microorganisms

The vehicle of any of the preceding claims for intranasal delivery of ammonia oxidizing microorganisms (AOM), wherein the preparation is of AOM.

The vehicle of any of the preceding claims, wherein the vacuum bag is substantially conical in geometry.

The vehicle of any of the preceding claims, wherein the vacuum bag includes 50 mL to 200 mL of the preparation.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to prevent clogging.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured as a nasal tip.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured for targeted delivery of the preparation to a nasal cavity of the subject.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver the preparation to a posterior region of the nasal cavity of the subject.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver a metered dose of the preparation to the subject.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver a predetermined volume of the preparation to the subject.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver a predetermined amount of nonpathogenic microorganisms to the subject.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver a predetermined amount of AOM to the subject.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver the preparation in a predetermined average particle size range.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver the preparation in a unidirectional flow from the vacuum bag.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver the preparation at an angle relative to an orientation of the end-use container.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver the preparation regardless of orientation of the end-use container.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to deliver the preparation when the end-use container is oriented upside down.

The vehicle of any of the preceding claims, wherein the spray nozzle includes an actuator.

The vehicle of any of the preceding claims, wherein the spray nozzle is pressure-actuated.

The vehicle of any of the preceding claims, wherein the spray nozzle includes an ejection port.

The vehicle of any of the preceding claims, wherein the ejection port is positioned above the actuator.

The vehicle of any of the preceding claims, wherein the spray nozzle is spring-loaded.

The vehicle of any of the preceding claims, wherein the spray nozzle is configured to not require re-priming.

The vehicle of any of the preceding claims, wherein the end-use container defines a venting hole.

The vehicle of any of the preceding claims, wherein the end-use container comprises a multilayer construction.

The vehicle of any of the preceding claims, wherein the multiple layers are co-extruded.

The vehicle of any of the preceding claims, wherein the end-use container comprises a filter in a flow path between the vacuum bag and the spray nozzle.

The vehicle of any of the preceding claims, wherein the filter comprises a membrane.

The vehicle of any of the preceding claims, wherein the end-use container comprises a neck oriented at an angle relative to a vertical axis of the end-use container.

The vehicle of any of the preceding claims, wherein the spray nozzle is positioned at a distal end of the neck.

The vehicle of any of the preceding claims, wherein the end-use container comprises a second reservoir.

The vehicle of any of the preceding claims, wherein the end-use container comprises a second vacuum bag.

The vehicle of any of the preceding claims, wherein the end-use container is configured to reduce retrograde flow.

The vehicle of any of the preceding claims, wherein the end-use container is defined by an aseptic interior.

The vehicle of any of the preceding claims, wherein the end-use container is substantially opaque.

The vehicle of any of the preceding claims, wherein the end-use container is substantially shatter-resistant.

The vehicle of any of the preceding claims, wherein a volume of the preparation is sufficient for clinical use.

The vehicle of any of the preceding claims, wherein a volume of the preparation is sufficient for commercial use.

The vehicle of any of the preceding claims, wherein a concentration of the preparation is sufficient for therapeutic use.

The vehicle of any of the preceding claims, wherein a concentration of the preparation is sufficient for cosmetic use.

The vehicle of any of the preceding claims, wherein the preparation comprises between about 1×103 CFU/mL to about 1×1014 CFU/mL cells.

The vehicle of any of the preceding claims, wherein the preparation comprises live nonpathogenic microorganisms.

The vehicle of any of the preceding claims, wherein the preparation comprises live AOM.

The vehicle of any of the preceding claims, wherein the preparation comprises a monoculture of a select community of nonpathogenic microorganisms.

The vehicle of any of the preceding claims, wherein the preparation comprises a monoculture of AOM.

The vehicle of any of the preceding claims, wherein the preparation comprises a monoculture of ammonia oxidizing bacteria (AOB).

The vehicle of any of the preceding claims, wherein the preparation comprises a monoculture of Nitrosomonas Eutropha.

The vehicle of any of the preceding claims, wherein the preparation is substantially free of a preservative.

The vehicle of any of the preceding claims, wherein the preparation comprises nonpathogenic microorganisms in a buffer solution, e.g., an aqueous buffer solution.

The vehicle of any of the preceding claims, wherein the preparation comprises AOM in a buffer solution, e.g., an aqueous buffer solution.

The vehicle of any of the preceding claims, wherein the buffer solution, e.g., aqueous buffer solution, comprises disodium phosphate and magnesium chloride, for example, 50 mM Na2HPO4 and 2 mM MgCl2 in water.

The vehicle of any of the preceding claims, wherein the buffer solution e.g., aqueous buffer solution, consisting essentially of disodium phosphate and magnesium chloride, for example, 50 mM Na2HPO4 and 2 mM MgCl2 in water.

The vehicle of any of the preceding claims, wherein the buffer solution, e.g., aqueous buffer solution, consists of disodium phosphate and magnesium chloride, for example, 50 mM Na2HPO4 and 2 mM MgCl2 in water.

The vehicle of any of the preceding claims, wherein the vehicle is configured to maintain pressure during operation.

The vehicle of any of the preceding claims, wherein the vehicle further comprises a nasal pump.

The vehicle of any of the preceding claims, wherein the vehicle is intended for clinical use.

The vehicle of any of the preceding claims, wherein the vehicle is intended for commercial use.

The vehicle of any of the preceding claims, wherein the vehicle further comprises instructions for its therapeutic use.

The vehicle of any of the preceding claims, wherein the vehicle further comprises instructions for its cosmetic use.

The vehicle of any of the preceding claims, wherein the vehicle further comprises instructions for its storage.

The vehicle of any of the preceding claims, wherein the vehicle includes an indication of a number of remaining administrations.

The vehicle of any of the preceding claims, wherein the vehicle includes a feature for counting the remaining volume of preparation or remaining number of administrations.

The vehicle of any of the preceding claims, wherein the count relates to volumetric measurement or number of administered sprays.

The vehicle of any of the preceding claims, wherein the vehicle further comprises a temperature sensor.

The vehicle of any of the preceding claims, wherein the vehicle further comprises an indication of viability of the nonpathogenic microorganisms.

The vehicle of any of the preceding claims, wherein the vehicle further comprises an indication of viability of the AOM.

The vehicle of any of the preceding claims, wherein the vehicle further comprises an indication of an anticipated expiration date.