US 2026/0166093 A1 · Rotator Cuff Therapy Using Muscle Fiber Fragments

Abstract

Methods and compositions are disclosed for repair of shoulder injuries by employing disaggregated muscle fiber fragments to regenerate functional shoulder muscle tissue. In some embodiments, the fragments retain functional satellite cells but exhibit cell wall rupture and have an average size of less than 150 μm. The methods include the preparation and implantation of compositions by extracting muscle tissue from a donor site, disaggregating muscle fibers from the extracted tissue, and fragmenting disaggregated muscle fibers into fiber fragments that exhibit cell wall rupture and preferably have an average size of less than 150 microns, more preferable less than about 100 microns, while retaining functional satellite cells. Upon injection, e.g., into the supraspinatus or other rotator cuff muscles, the muscle fiber fragment compositions are capable of reconstituting or reconstructing elongated muscle fibers from the fragments and orienting in alignment with native shoulder muscle fibers.

Claims20

Dependent claims

A method of augmenting rotator cuff repair surgery on a patient comprising:

selecting a patient in need of rotator cuff repair surgery;
preparing a muscle regenerating composition for shoulder injury repair for the patient in need thereof comprising a population of muscle fiber fragments obtained from the pectoral muscle of the patient by a method comprising:
a) extracting autologous muscle tissue from a donor site of the patient's pectoralis major muscle;
b) disaggregating muscle fibers from the extracted tissue;
c) fragmenting disaggregated muscle fibers into fiber fragments that exhibit cell wall rupture and have an average size of less than 150 μm;
and
d) suspending the muscle fiber fragments in phosphate buffered saline to form the muscle regenerating composition,
e) performing a rotator cuff repair surgery on the patient;
and
f) injecting the muscle regenerating composition into a target muscle site proximal to the rotator cuff,
wherein the composition reconstructs elongated muscle fibers from the fragments and orients them in alignment with the native muscle fibers when implanted in the target muscle site proximal to the rotator cuff, thereby augmenting rotator cuff repair surgery in the patient.

8 more elements

Dependencies are inferred from the claim text.

Rotator Cuff Therapy Using Muscle Fiber Fragments

Life of this patent

Rotator Cuff Therapy Using Muscle Fiber Fragments

Life of this patent

Abstract

Claims20