Single-Cell Microsphere Prepared Based on Oil-Free Aqueous Two-Phase System Strategy and Preparation Method Therefor

Abstract

The present invention discloses a single-cell microsphere prepared based on an oil-free aqueous two-phase system strategy and a preparation method therefor. Specifically, through gravity induction, cells in an upper aqueous phase enter a lower aqueous phase; due to adhesion between the cells and hydrogel and a stable interface formed by two aqueous phases, the individual cells entering the lower aqueous phase are encapsulated by the hydrogel. Being free of oils and surfactants, the method ensures high cell viability and enables universal encapsulation across different cell types.

Dependencies are inferred from the claim text.