Methods for sex-sorting insects

Abstract

Provided herein are methods of sex-sorting a plurality of insects based on sex-specific gene expression, the method comprising (a) generating an exogenous nucleic acid molecule; (b) delivering the exogenous nucleic acid molecule into an insect from the plurality of insects, wherein the exogenous nucleic acid molecule comprises a promoter region, a sex-specific splicing module, a reporter gene, and a transcription terminator; (c) detecting sex-specific gene expression of the reporter gene; and (d) sorting the insect from the plurality of insects based on the detecting of the sex-specific gene expression in step (c), thereby sex-sorting the insect based on the sex-specific gene expression.

Dependencies are inferred from the claim text.