High-throughput protein detection method based on adjacent dna encoding

A method for detecting a protein, the method comprising:

• mixing a first probe, a second probe, and a third probe with a protein to be detected, to obtain a sequencing library;
• and
• sequencing the sequencing library for detecting the protein to be detected, wherein:
• the first probe comprises a first oligonucleotide strand and a first antibody, wherein the 5′ end of the first oligonucleotide strand is linked to the first antibody, and wherein the first antibody has an activity of binding to a predetermined protein;
• the second probe comprises a second oligonucleotide strand and a second antibody, wherein the 5′ end of the second oligonucleotide strand is linked to the second antibody, wherein the second antibody has an activity of binding to the predetermined protein, and wherein at least a partial sequence at the 3′ end of the first oligonucleotide strand is capable of reverse complementary pairing with at least a partial sequence at the 3′ end of the second oligonucleotide strand;
• and
• the third probe comprises a third oligonucleotide strand, wherein at least a partial sequence at the 5′ end of the third oligonucleotide strand is complementarily paired with a partial sequence at the 3′ end of the second oligonucleotide strand.