Method for preparing circular rna using dumbbell structure

Abstract

The present disclosure relates to a circular RNA precursor having a dumbbell-shaped secondary structure, and the like. According to the present disclosure, the circular RNA precursor uses T4 RNA ligase 2 (Rnl2), but does not require a DNA splint in preparing circular RNA, and furthermore, it is possible to prepare circular RNA with greater efficiency than using the DNA splint. In addition, in preparation of circular RNA using a ribozyme, the ribozyme needs to be finally removed, but in the present disclosure, an additional RNA removal step is not required. In addition, the circular RNA precursor of the present disclosure may be prepared as circular RNA containing a protein-encoding gene as GOI, and thus can be used as a gene therapy for the purpose of protein expression.

Dependencies are inferred from the claim text.