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US 2026/0158190 A1Published application
Bioprosthetic tissue preparation
AssigneeEdwards Lifesciences Corporation
InventorsBin Tian, Angela B. De La Fuente, Gregory A. Wright, Jingjia Han, Hao Shang
Life of this patent
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Abstract
Methods for preparing bioprosthetic tissue are provided. In some instances, bioprosthetic tissue is treated to remove antigenic biomolecules. In some instances, prepared bioprosthetic tissues are incorporated into a medical device.
Claims21
A method to prepare bioprosthetic tissue for clinical or preclinical use, comprising:
- (a) treating animal tissue with one or more of the following:
- a reducing agent, a zwitterionic detergent, and a nuclease;
- (b) treating the animal tissue with a solution comprising glycerol and a C1-C3 alcohol;
- (c) treating the animal tissue with a crosslinking agent;
- (d) equilibrating the animal tissue with a biocompatible molecule;
- and
- (e) storing the animal tissue with the biocompatible molecule equilibrated therein and free of liquids;
- wherein step c is to be performed prior to steps d and e.
5 more elements
A method to prepare bioprosthetic tissue for clinical or preclinical use, comprising:
- (a) treating animal tissue with a solution comprising a reducing agent, wherein the reducing agent is:
- β-mercaptoethanol (BME or 2-ME), tris(2-carboxyethyl)phosphine (TCEP), dithiothreitol (DTT), or tributylphosphine (TEP or TnBP);
- (b) treating the animal tissue with a crosslinking agent;
- and
- (c) treating the bioprosthetic tissue with a solution for dry storage or wet storage, wherein the solution for dry storage comprises a glycerol, polyethylene glycol, or a saccharide, wherein the solution for wet storage comprises glutaraldehyde or propylene oxide;
- wherein steps a and b are to be performed prior to step c.
3 more elements
A method to prepare bioprosthetic tissue for clinical or preclinical use, comprising:
- (a) treating animal tissue with a solution comprising a nuclease;
- (b) treating the animal tissue with a crosslinking agent;
- and
- (c) treating the bioprosthetic tissue with a solution for dry storage or wet storage, wherein the solution for dry storage comprises a glycerol, polyethylene glycol, or a saccharide, wherein the solution for wet storage comprises glutaraldehyde or propylene oxide;
- wherein steps a and b are to be performed prior to step c.
2 more elements
Dependencies are inferred from the claim text.