GREENHOUSE CULTIVATION METHOD FOR MOREL (MORCHELLA Spp.) USING LIQUID SPAWN

A greenhouse cultivation method for morels using liquid spawn, comprising the following steps:

• step 1:
• preparing culture medium
• preparing PDA (Potato Dextrose Agar) culture medium, liquid culture medium and exogenous nutrient bags;
• step 2:
• purifying spawn
• inoculating morel mother spawn onto the PDA medium for activation cultivation in a dark condition. After a period of time, selecting uncontaminated PDA medium and transferring the mycelia to a fresh PDA medium for further purification cultivation in the dark environment;
• step 3:
• preparing liquid spawn
• selecting the uncontaminated PDA medium when the mycelia have fully colonized the PDA medium, transferring the mycelia from the uncontaminated PDA medium into CYM (Complete Yeast Medium) liquid medium for cultivation, and obtaining the liquid spawn when the mycelia fully colonized the CYM liquid medium;
• step 4:
• sowing
• plowing soil and making cultivation beds when the greenhouse temperature ranges from above 0° C. to 20° C. (0° C. <temperature ≤20° C.) and the soil pH is between 6.5-7.5, diluting the liquid spawn with water and evenly spreading it on the raised beds surface in an amount of 140-200L /mu, then irrigating until the soil moisture content reaches 20-25%, 7-10 d after sowing, placing exogenous nutrient bags in a way that allows the mycelia contact them;
• step 5, performing management during growth period
• management during spawn-running period:
• maintaining an air temperature ≤20° C., an air relative humidity at 80-95%, a soil temperature ≤15° C., and a soil moisture content at 15-25%;
• management during mushroom induction period:
• maintaining an air humidity at 85-95%, and the soil moisture content at 25-30%;
• 40-80 d after morel sowing, when the soil temperature maintained between 8-15° C., applying water stimulation;
• management from primordium differentiation to young mushroom stage:
• maintaining the air temperature at 4-18° C. and the relative air humidity >85%;
• management during fruit body growth period:
• after the formation of 1.5-3 cm small mushrooms, maintaining the air temperature at 10-18° C., the air relative humidity at 80-90%, and the soil moisture content at 20-25%;
• management during fruiting body maturation stage:
• maintaining the air relative humidity at 70-85%, and reducing the soil moisture content to 18-22%;
• step 6, harvesting morels when the total length of fruiting bodies reaches 12-14 cm with the pileus length of 7-9 cm.