Compositions for delivery of plasmodium csp antigens and related methods

An RNA construct comprising in 5′ to 3′ order:

• (i) a 5′ UTR that comprises or consists of a modified human alpha-globin 5′-UTR;
• (ii) a polyribonucleotide encoding a polypeptide that comprises the amino acid sequence of SEQ ID NO:
• 1 or an amino acid sequence having at least 98% or at least 99% sequence identity to an amino acid sequence according to SEQ ID NO:
• 1;
• (iii) a 3′ UTR that comprises or consists of a first sequence from the amino terminal enhancer of split
• (AES) messenger RNA and a second sequence from the mitochondrial encoded 125 ribosomal RNA;
• and
• (iv) a polyA tail sequence.