US 2026/0144184 A1Published application

Methods for improved hybrid corn seed production

AssigneeMonsanto Technology LLC

InventorsPaul Cannon, Edward J. Cargill, Charles T. Foresman, Michael A. Hall, Scott C. Johnson, John A. Miklos

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Abstract

Methods for improving the efficiency and productivity of hybrid corn seed production are provided herein. Various methods to improve the transfer of pollen from male corn plants to female corn plants, and thus increase yield, are provided herein. Without being limiting, these methods include varying the height of male and female corn plants in a field, as well as varying the number, arrangement, and ratio of male-to-female rows in a field.

Claims27

Dependent claims

A method comprising:

(a) fertilizing a plurality of female inbred corn plants with pollen from at least one male inbred corn plant to produce hybrid corn seeds, wherein the female inbred corn plants have an average height that is at least 2.5% lower than the average height of the at least one male inbred corn plant, and wherein the female inbred corn plants comprise:
(i) a mutant allele of an endogenous GA20 oxidase_3 locus, wherein the mutant allele comprises a first DNA segment inserted into the endogenous GA20 oxidase_3 locus, wherein the first DNA segment encodes an antisense RNA sequence that is at least 70% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
182-184 and 186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3 locus produces a RNA transcript comprising the antisense RNA sequence;
or
(ii) a first mutant allele of an endogenous GA20 oxidase_5 locus, wherein the first mutant allele of the endogenous GA20 oxidase_5 locus comprises a second DNA segment inserted into the endogenous GA20 oxidase_5 locus, wherein the second DNA segment encodes an antisense RNA sequence that is at least 70% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
182-184 and 186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5 locus produces a RNA transcript comprising the antisense RNA sequence;
or
(iii) a first mutant allele of an endogenous Brachytic2 (br2) locus, wherein the first mutant allele of an endogenous br2 locus comprises a third DNA segment inserted into the endogenous br2 locus, wherein the third DNA segment encodes an antisense RNA that is at least 70% complementary to at least 20 consecutive nucleotides of SEQ ID NO:
132 or 180, and wherein the mutant allele of the endogenous br2 locus produces an RNA transcript comprising the antisense RNA sequence;
or
(iv) a second mutant allele of an endogenous br2 locus, wherein the second mutant allele of an endogenous br2 locus comprises a deletion of at least one nucleotide from an endogenous br2 locus as compared to SEQ ID NO:
132;
or
(v) a dominant or semi-dominant transgene or mutant allele of a gene, and wherein the transgene or mutant allele causes a short stature phenotype in the at least one corn plant;
or
(vi) a premature stop codon within a nucleic acid sequence encoding a Brachytic2 protein as compared to a control corn plant;
or
(vii) a second mutant allele of an endogenous GA20 oxidase_5 locus, wherein the second mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification comprising a deletion of at least a portion of the transcription termination sequence of the endogenous Zm.SAMT gene, and wherein the mutant allele produces a RNA molecule comprising an antisense sequence complementary to all or part of the sense strand of the endogenous GA20 oxidase_5 gene;
or
(viii) a third mutant allele of an endogenous GA20 oxidase_5 locus, wherein the third mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification comprising a deletion of at least a portion of the intergenic region between the endogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutant allele produces a RNA molecule comprising an antisense sequence complementary to all or part of the sense strand of the endogenous GA20 oxidase_5 gene;
or
(ix) a genome modification comprising a deletion of at least a portion of one or more of the following:
5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any portion thereof, of the endogenous Zm.SAMT gene;
or
(x) a fourth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the fourth mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification which results in the transcription of an antisense strand of at least an exon, an intron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof, or
(xi) a fifth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the fifth mutant allele of the endogenous GA20 oxidase_5 locus comprises the Zm.SAMT gene promoter, or a functional part thereof, operably linked to at least one transcribable antisense sequence of at least an exon, intron or untranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof, or
(xii) a sixth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the sixth mutant allele of the endogenous GA20 oxidase_5 locus comprises a sequence selected from the group consisting of SEQ ID NOs:
87-105;
or
(xiii) a seventh mutant allele of an endogenous GA20 oxidase_5 locus, wherein the seventh mutant allele of the endogenous GA20 oxidase_5 locus comprises a first sequence and a second sequence;
wherein the first sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;
and wherein the second sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.SAMT gene;
wherein the first sequence and the second sequence are contiguous or separated only by an intervening sequence of fewer than 555 nucleotides;
or
(xiv) an eighth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the eighth mutant allele of the endogenous GA20 oxidase_5 locus comprises a genomic deletion relative to a wild type allele of the endogenous GA20 oxidase_5 locus, wherein the genomic deletion is flanked by a first sequence and a second sequence;
wherein the first sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;
and wherein the second sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.SAMT gene;
or
(xv) a genomic sequence comprising a first sequence and a second sequence;
wherein the first sequence comprises at least 15 consecutive nucleotides of one or more of SEQ ID NOs:
228-235 and 276-283;
wherein the second sequence comprises at least 15 consecutive nucleotides of one or more of SEQ ID NOs:
235-276;
and wherein the genomic sequence is at least 50 consecutive nucleotides in length, and/or fewer than 9000 consecutive nucleotides in length;
or
(xvi) a recombinant DNA construct comprising a transcribable DNA sequence encoding a GA2 oxidase protein and a plant-expressible promoter, wherein the transcribable DNA sequence is operably linked to the plant-expressible promoter;
and
(b) harvesting said hybrid corn seeds from one or more of the female inbred corn plants.

45 more elements

A method comprising:

(a) crossing at least one male inbred corn plant with a plurality of female inbred corn plants to produce hybrid corn seeds, wherein the plurality of female inbred corn plants have an average height that is at least 2.5% lower than the height or average height of the at least one male inbred corn plant, and wherein the female inbred corn plant comprises:
(i) a mutant allele of an endogenous GA20 oxidase_3 locus, wherein the mutant allele comprises a first DNA segment inserted into the endogenous GA20 oxidase_3 locus, wherein the first DNA segment encodes an antisense RNA sequence that is at least 70% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
182-184 and 186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3 locus produces a RNA transcript comprising the antisense RNA sequence;
or
(ii) a first mutant allele of an endogenous GA20 oxidase_5 locus, wherein the first mutant allele of the endogenous GA20 oxidase_5 locus comprises a second DNA segment inserted into the endogenous GA20 oxidase_5 locus, wherein the second DNA segment encodes an antisense RNA sequence that is at least 70% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
182-184 and 186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5 locus produces a RNA transcript comprising the antisense RNA sequence;
or
(iii) a first mutant allele of an endogenous Brachytic2 (br2) locus, wherein the first mutant allele of an endogenous br2 locus comprises a third DNA segment inserted into the endogenous br2 locus, wherein the third DNA segment encodes an antisense RNA that is at least 70% complementary to at least 20 consecutive nucleotides of SEQ ID NO:
132 or 180, and wherein the mutant allele of the endogenous br2 locus produces an RNA transcript comprising the antisense RNA sequence;
or
(iv) a second mutant allele of an endogenous br2 locus, wherein the second mutant allele of an endogenous br2 locus comprises a deletion of at least one nucleotide from an endogenous br2 locus as compared to SEQ ID NO:
132;
or
(v) a dominant or semi-dominant transgene or mutant allele of a gene, and wherein the transgene or mutant allele causes a short stature phenotype in the at least one corn plant;
or
(vi) a premature stop codon within a nucleic acid sequence encoding a Brachytic2 protein as compared to a control corn plant;
or
(vii) a second mutant allele of an endogenous GA20 oxidase_5 locus, wherein the second mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification comprising a deletion of at least a portion of the transcription termination sequence of the endogenous Zm.SAMT gene, and wherein the mutant allele produces a RNA molecule comprising an antisense sequence complementary to all or part of the sense strand of the endogenous GA20 oxidase_5 gene;
or
(viii) a third mutant allele of an endogenous GA20 oxidase_5 locus, wherein the third mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification comprising a deletion of at least a portion of the intergenic region between the endogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutant allele produces a RNA molecule comprising an antisense sequence complementary to all or part of the sense strand of the endogenous GA20 oxidase_5 gene;
or
(ix) a genome modification comprising a deletion of at least a portion of one or more of the following:
5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any portion thereof, of the endogenous Zm.SAMT gene;
or
(x) a fourth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the fourth mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification which results in the transcription of an antisense strand of at least an exon, an intron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof, or
(xi) a fifth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the fifth mutant allele of the endogenous GA20 oxidase_5 locus comprises the Zm.SAMT gene promoter, or a functional part thereof, operably linked to at least one transcribable antisense sequence of at least an exon, intron or untranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof, or
(xii) a sixth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the sixth mutant allele of the endogenous GA20 oxidase_5 locus comprises a sequence selected from the group consisting of SEQ ID NOs:
87-105;
or
(xiii) a seventh mutant allele of an endogenous GA20 oxidase_5 locus, wherein the seventh mutant allele of the endogenous GA20 oxidase_5 locus comprises a first sequence and a second sequence;
wherein the first sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;
and wherein the second sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.SAMT gene;
wherein the first sequence and the second sequence are contiguous or separated only by an intervening sequence of fewer than 555 nucleotides;
or
(xiv) an eighth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the eighth mutant allele of the endogenous GA20 oxidase_5 locus comprises a genomic deletion relative to a wild type allele of the endogenous GA20 oxidase_5 locus, wherein the genomic deletion is flanked by a first sequence and a second sequence;
wherein the first sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;
and wherein the second sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.SAMT gene;
or
(xv) a genomic sequence comprising a first sequence and a second sequence;
wherein the first sequence comprises at least 15 consecutive nucleotides of one or more of SEQ ID NOs:
228-235 and 276-283;
wherein the second sequence comprises at least 15 consecutive nucleotides of one or more of SEQ ID NOs:
235-276;
and wherein the genomic sequence is at least 50 consecutive nucleotides in length, and/or fewer than 9000 consecutive nucleotides in length;
or
(xvi) a recombinant DNA construct comprising a transcribable DNA sequence encoding a GA2 oxidase protein and a plant-expressible promoter, wherein the transcribable DNA sequence is operably linked to the plant-expressible promoter;
and
(b) harvesting said hybrid corn seeds from one or more of the female inbred corn plants.

45 more elements

A method comprising:

(a) planting at least one male inbred corn plant in proximity to a plurality of female inbred corn plants to produce hybrid corn seeds, wherein the female inbred corn plants have an average height that is at least 2.5% lower than the height or average height of the at least one male inbred corn plant, and wherein the female inbred corn plant comprises:
(i) a mutant allele of an endogenous GA20 oxidase_3 locus, wherein the mutant allele comprises a first DNA segment inserted into the endogenous GA20 oxidase_3 locus, wherein the first DNA segment encodes an antisense RNA sequence that is at least 70% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
182-184 and 186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3 locus produces a RNA transcript comprising the antisense RNA sequence;
or
(ii) a first mutant allele of an endogenous GA20 oxidase_5 locus, wherein the first mutant allele of the endogenous GA20 oxidase_5 locus comprises a second DNA segment inserted into the endogenous GA20 oxidase_5 locus, wherein the second DNA segment encodes an antisense RNA sequence that is at least 70% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
182-184 and 186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5 locus produces a RNA transcript comprising the antisense RNA sequence;
or
(iii) a first mutant allele of an endogenous Brachytic2 (br2) locus, wherein the first mutant allele of an endogenous br2 locus comprises a third DNA segment inserted into the endogenous br2 locus, wherein the third DNA segment encodes an antisense RNA that is at least 70% complementary to at least 20 consecutive nucleotides of SEQ ID NO:
132 or 180, and wherein the mutant allele of the endogenous br2 locus produces an RNA transcript comprising the antisense RNA sequence;
or
(iv) a second mutant allele of an endogenous br2 locus, wherein the second mutant allele of an endogenous br2 locus comprises a deletion of at least one nucleotide from an endogenous br2 locus as compared to SEQ ID NO:
132;
or
(v) a dominant or semi-dominant transgene or mutant allele of a gene, and wherein the transgene or mutant allele causes a short stature phenotype in the at least one corn plant;
or
(vi) a premature stop codon within a nucleic acid sequence encoding a Brachytic2 protein as compared to a control corn plant;
or
(vii) a second mutant allele of an endogenous GA20 oxidase_5 locus, wherein the second mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification comprising a deletion of at least a portion of the transcription termination sequence of the endogenous Zm.SAMT gene, and wherein the mutant allele produces a RNA molecule comprising an antisense sequence complementary to all or part of the sense strand of the endogenous GA20 oxidase_5 gene;
or
(viii) a third mutant allele of an endogenous GA20 oxidase_5 locus, wherein the third mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification comprising a deletion of at least a portion of the intergenic region between the endogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutant allele produces a RNA molecule comprising an antisense sequence complementary to all or part of the sense strand of the endogenous GA20 oxidase_5 gene;
or
(ix) a genome modification comprising a deletion of at least a portion of one or more of the following:
5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any portion thereof, of the endogenous Zm.SAMT gene;
or
(x) a fourth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the fourth mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification which results in the transcription of an antisense strand of at least an exon, an intron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof, or
(xi) a fifth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the fifth mutant allele of the endogenous GA20 oxidase_5 locus comprises the Zm.SAMT gene promoter, or a functional part thereof, operably linked to at least one transcribable antisense sequence of at least an exon, intron or untranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof, or
(xii) a sixth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the sixth mutant allele of the endogenous GA20 oxidase_5 locus comprises a sequence selected from the group consisting of SEQ ID NOs:
87-105;
or
(xiii) a seventh mutant allele of an endogenous GA20 oxidase_5 locus, wherein the seventh mutant allele of the endogenous GA20 oxidase_5 locus comprises a first sequence and a second sequence;
wherein the first sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;
and wherein the second sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.SAMT gene;
wherein the first sequence and the second sequence are contiguous or separated only by an intervening sequence of fewer than 555 nucleotides;
or
(xiv) an eighth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the eighth mutant allele of the endogenous GA20 oxidase_5 locus comprises a genomic deletion relative to a wild type allele of the endogenous GA20 oxidase_5 locus, wherein the genomic deletion is flanked by a first sequence and a second sequence;
wherein the first sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;
and wherein the second sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.SAMT gene;
or
(xv) a genomic sequence comprising a first sequence and a second sequence;
wherein the first sequence comprises at least 15 consecutive nucleotides of one or more of SEQ ID NOs:
228-235 and 276-283;
wherein the second sequence comprises at least 15 consecutive nucleotides of one or more of SEQ ID NOs:
235-276;
and wherein the genomic sequence is at least 50 consecutive nucleotides in length, and/or fewer than 9000 consecutive nucleotides in length;
or
(xvi) a recombinant DNA construct comprising a transcribable DNA sequence encoding a GA2 oxidase protein and a plant-expressible promoter, wherein the transcribable DNA sequence is operably linked to the plant-expressible promoter;
and
(b) harvesting said hybrid corn seeds from one or more of the female inbred corn plants.

45 more elements

Dependencies are inferred from the claim text.

Facts

Application: 19/425,440Filed 18 Dec 2025
Priority: 23 Nov 2020
Published: 28 May 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification19 codes

A01C 21/00
Methods of fertilising , sowing or planting
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
A01D 91/04
Methods for harvesting agricultural products: products growing above the soil
A01G 22/20
Cultivation of specific crops or plants not otherwise provided for: cereals

Family

Application63/117,231Us provisional application · filed 23 Nov 2020
Application63/117,237Us provisional application · filed 23 Nov 2020
Application63/117,247Us provisional application · filed 23 Nov 2020
Application63/117,225Us provisional application · filed 23 Nov 2020
Application63/125,752Us provisional application · filed 15 Dec 2020
Application63/180,344Us provisional application · filed 27 Apr 2021
Application17/530,873Division · filed 19 Nov 2021
Application19/425,440Division
US 12,527,250 B2Related patent
US 2022/0159919 A1Published application
US 2026/0144184 A1This patent

From the US filing history. Foreign family members aren’t listed.

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Abstract

A method comprising:

(a) fertilizing a plurality of female inbred corn plants with pollen from at least one male inbred corn plant to produce hybrid corn seeds, wherein the female inbred corn plants have an average height that is at least 2.5% lower than the average height of the at least one male inbred corn plant, and wherein the female inbred corn plants comprise:
(i) a mutant allele of an endogenous GA20 oxidase_3 locus, wherein the mutant allele comprises a first DNA segment inserted into the endogenous GA20 oxidase_3 locus, wherein the first DNA segment encodes an antisense RNA sequence that is at least 70% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
182-184 and 186-188, and wherein the mutant allele of the endogenous GA20 oxidase_3 locus produces a RNA transcript comprising the antisense RNA sequence;
or
(ii) a first mutant allele of an endogenous GA20 oxidase_5 locus, wherein the first mutant allele of the endogenous GA20 oxidase_5 locus comprises a second DNA segment inserted into the endogenous GA20 oxidase_5 locus, wherein the second DNA segment encodes an antisense RNA sequence that is at least 70% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
182-184 and 186-188, and wherein the mutant allele of the endogenous GA20 oxidase_5 locus produces a RNA transcript comprising the antisense RNA sequence;
or
(iii) a first mutant allele of an endogenous Brachytic2 (br2) locus, wherein the first mutant allele of an endogenous br2 locus comprises a third DNA segment inserted into the endogenous br2 locus, wherein the third DNA segment encodes an antisense RNA that is at least 70% complementary to at least 20 consecutive nucleotides of SEQ ID NO:
132 or 180, and wherein the mutant allele of the endogenous br2 locus produces an RNA transcript comprising the antisense RNA sequence;
or
(iv) a second mutant allele of an endogenous br2 locus, wherein the second mutant allele of an endogenous br2 locus comprises a deletion of at least one nucleotide from an endogenous br2 locus as compared to SEQ ID NO:
132;
or
(v) a dominant or semi-dominant transgene or mutant allele of a gene, and wherein the transgene or mutant allele causes a short stature phenotype in the at least one corn plant;
or
(vi) a premature stop codon within a nucleic acid sequence encoding a Brachytic2 protein as compared to a control corn plant;
or
(vii) a second mutant allele of an endogenous GA20 oxidase_5 locus, wherein the second mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification comprising a deletion of at least a portion of the transcription termination sequence of the endogenous Zm.SAMT gene, and wherein the mutant allele produces a RNA molecule comprising an antisense sequence complementary to all or part of the sense strand of the endogenous GA20 oxidase_5 gene;
or
(viii) a third mutant allele of an endogenous GA20 oxidase_5 locus, wherein the third mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification comprising a deletion of at least a portion of the intergenic region between the endogenous GA20 oxidase_5 and Zm.SAMT genes, and wherein the mutant allele produces a RNA molecule comprising an antisense sequence complementary to all or part of the sense strand of the endogenous GA20 oxidase_5 gene;
or
(ix) a genome modification comprising a deletion of at least a portion of one or more of the following:
5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any portion thereof, and the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any portion thereof, of the endogenous Zm.SAMT gene;
or
(x) a fourth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the fourth mutant allele of the endogenous GA20 oxidase_5 locus comprises a genome modification which results in the transcription of an antisense strand of at least an exon, an intron, or an untranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof, or
(xi) a fifth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the fifth mutant allele of the endogenous GA20 oxidase_5 locus comprises the Zm.SAMT gene promoter, or a functional part thereof, operably linked to at least one transcribable antisense sequence of at least an exon, intron or untranslated region (UTR) of the endogenous GA20 oxidase_5 gene, or any portion thereof, or
(xii) a sixth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the sixth mutant allele of the endogenous GA20 oxidase_5 locus comprises a sequence selected from the group consisting of SEQ ID NOs:
87-105;
or
(xiii) a seventh mutant allele of an endogenous GA20 oxidase_5 locus, wherein the seventh mutant allele of the endogenous GA20 oxidase_5 locus comprises a first sequence and a second sequence;
wherein the first sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;
and wherein the second sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.SAMT gene;
wherein the first sequence and the second sequence are contiguous or separated only by an intervening sequence of fewer than 555 nucleotides;
or
(xiv) an eighth mutant allele of an endogenous GA20 oxidase_5 locus, wherein the eighth mutant allele of the endogenous GA20 oxidase_5 locus comprises a genomic deletion relative to a wild type allele of the endogenous GA20 oxidase_5 locus, wherein the genomic deletion is flanked by a first sequence and a second sequence;
wherein the first sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.GA20 oxidase_5 gene;
and wherein the second sequence comprises one or more of the 5′ UTR, 1st exon, 1st intron, 2nd exon, 2nd intron, 3rd exon, 3rd intron, 4th exon, 4th intron, 5th exon, 5th intron, 6th exon, 6th intron, 7th exon, 7th intron, 8th exon, 3′ UTR, and any complementary sequence thereof, and any portion of the foregoing, of the endogenous Zm.SAMT gene;
or
(xv) a genomic sequence comprising a first sequence and a second sequence;
wherein the first sequence comprises at least 15 consecutive nucleotides of one or more of SEQ ID NOs:
228-235 and 276-283;
wherein the second sequence comprises at least 15 consecutive nucleotides of one or more of SEQ ID NOs:
235-276;
and wherein the genomic sequence is at least 50 consecutive nucleotides in length, and/or fewer than 9000 consecutive nucleotides in length;
or
(xvi) a recombinant DNA construct comprising a transcribable DNA sequence encoding a GA2 oxidase protein and a plant-expressible promoter, wherein the transcribable DNA sequence is operably linked to the plant-expressible promoter;
and
(b) harvesting said hybrid corn seeds from one or more of the female inbred corn plants.

45 more elements

Facts

Application

19/425,440Filed 18 Dec 2025

Priority

23 Nov 2020

Published

28 May 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification19 codes

A01C 21/00
Methods of fertilising , sowing or planting
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
A01D 91/04
Methods for harvesting agricultural products: products growing above the soil
A01G 22/20
Cultivation of specific crops or plants not otherwise provided for: cereals

Family

Application63/117,231Us provisional application · filed 23 Nov 2020

Application63/117,237Us provisional application · filed 23 Nov 2020

Application63/117,247Us provisional application · filed 23 Nov 2020

Application63/117,225Us provisional application · filed 23 Nov 2020

Application63/125,752Us provisional application · filed 15 Dec 2020

Application63/180,344Us provisional application · filed 27 Apr 2021

Application17/530,873Division · filed 19 Nov 2021

Application19/425,440Division

US 12,527,250 B2Related patent

US 2022/0159919 A1Published application

US 2026/0144184 A1This patent

From the US filing history. Foreign family members aren’t listed.