Transcriptional regulators and polynucleotides encoding the same

18:

• A fungal host cell comprising in its genome at least one first heterologous promoter operably linked to a first polynucleotide encoding a fungal transcriptional regulator polypeptide, or variant thereof, comprising or consisting of an amino acid sequence having a sequence identity of at least 80% to SEQ ID NO:
• 24.

19:

• The fungal host cell according to claim 18, wherein the transcriptional regulator polypeptide, or variant thereof, is endogenous to the host cell.

20:

• The fungal host cell according to claim 18, wherein the transcriptional regulator polypeptide, or variant thereof, comprises at least one DNA-binding motif comprising or consisting of an amino acid sequence having a sequence identity of at least 80% to SEQ ID NO:79 or SEQ ID NO:
• 80.

21:

• The fungal host cell according to claim 18, wherein the transcriptional regulator polypeptide comprises or consists of an amino acid sequence of SEQ ID NO:
• 24.

22:

• The fungal host cell according to claim 18, comprising in its genome at least one second heterologous promoter operably linked to at least one second polynucleotide encoding at least one polypeptide of interest.

23:

• The fungal host cell according to claim 22, wherein the at least one polypeptide of interest is secreted.

24:

• The fungal host cell according to claim 18, wherein the first heterologous promoter operably linked to the first polynucleotide is endogenous to the host cell.

25:

• The fungal host cell according to claim 18, wherein the host cell is a filamentous fungal host cell.

26:

• The fungal host cell according to claim 25, wherein the filamentous fungal host cell is a Trichoderma reesei cell.

27:

• The fungal host cell according to claim 18, wherein the host cell is a yeast host cell.

28:

• The fungal host cell according to claim 18, wherein the at least one polypeptide of interest comprises a heme-containing polypeptide, a brazzein, a casein, a patatin, an ovalbumin, an osteopontin, an ovotransferrin, an ovomucin, an ovomucoid, an ovostatin, a lactoferrin, an alpha-lactalbumin, a beta-lactalbumin, a glycomacropeptide, a collagen, and/or a therapeutic polypeptide.

29:

• The fungal host cell according to claim 18, wherein the at least one polypeptide of interest is selected from the group consisting of a hydrolase, isomerase, ligase, lyase, lysozyme, oxidoreductase, or transferase.

30:

• The fungal host cell according to claim 18, wherein the at least one polypeptide of interest is a glycosylase.

33:

• A nucleic acid construct comprising at least one first heterologous promoter operably linked to a first polynucleotide encoding a fungal transcriptional regulator polypeptide, or variant thereof, comprising or consisting of an amino acid sequence having a sequence identity of at least 80% to SEQ ID NO:24.

34:

• An expression vector comprising a nucleic acid construct according to claim 33.