De novo synthesized gene libraries

A method for polynucleotide synthesis, comprising:

• receiving first instructions in a computer readable non-transient medium for synthesis of DNA sequences, wherein each DNA sequence has a full length sequence of 100 bases to 350 bases in length;
• processing the first instructions in a computer and transmitting second instructions to a material deposition device, wherein the second instructions provide for synthesis of a plurality of polynucleotides, wherein the plurality of polynucleotides comprise the DNA sequences;
• and
• releasing synthesis reagents from the material deposition device to synthesize the plurality of polynucleotides, wherein 67% or more of the plurality of polynucleotides that are synthesized encode for the full length sequence of the DNA sequences compared to the DNA sequences received in the first instructions.