Probiotic lactobacillus strain compositions and applications therefor including for leaky gut and inflammation

A method for reducing intestinal permeability, alleviating alcohol induced or disease related leaky gut syndrome, alleviating the intestinal and systemic effects of chronic alcohol consumption, including intestinal dysbiosis, and/or reducing inflammation, including intestinal-derived or intestinal-associated inflammation, comprising administering an effective amount of an immunogenic probiotic composition comprising at least one of an isolated first Lactobacillus reuteri strain and an isolated second Lactobacillus reuteri strain wherein:

• (a) the isolated first Lactobacillus reuteri strain has a genomic nucleic acid sequence including at least one of SEQ ID NOs:
• 49-55, sequences having one or more nucleic acid sequence difference from the sequence of at least one of SEQ ID NOs:
• 49-55, sequences having at least one nucleic acid sequence difference from the sequence of at least one of SEQ ID NOs:
• 49-55 and further having at least 97%, at least 98%, at least 99% or at least 99.5% sequence identity with one or more of SEQ ID NOs:
• 49-55, sequences having at least 98%, at least 98.5%, at least 99%, or at least 99.5% sequence identity to SEQ ID NOs:
• 49-55, and/or
• the isolated second Lactobacillus reuteri strain has a genomic nucleic acid sequence including at least one of SEQ ID NOs:
• 44-48, sequences having one or more nucleic acid sequence difference from the sequence of at least one of SEQ ID NOs:
• 44-48, sequences having at least one nucleic acid sequence difference from the sequence of at least one of SEQ ID NOs:
• 44-48 and further having at least 97%, at least 98%, at least 99% or at least 99.5% sequence identity with one or more of SEQ ID NOs:
• 44-48, sequences having at least 98%, at least 98.5%, at least 99%, or at least 99.5% sequence identity to SEQ ID NOs:
• 44-48;
• or
• (b) the isolated first Lactobacillus reuteri strain comprises or has a genomic nucleic acid sequence corresponding to the genomic nucleic acid sequence of ATCC strain PTA-126788, or a variant thereof comprising or having a nucleic acid sequence at least 98%, at least 98.5%, at least 99%, or at least 99.5% sequence identity to the genomic nucleic acid sequence of ATCC strain PTA-126788, or is Lactobacillus reuteri strain 3632, which corresponds to ATCC Patent Deposit Number PTA-126788;
• and/or
• the isolated second Lactobacillus reuteri strain comprises or has a genomic nucleic acid sequence corresponding to the genomic nucleic acid sequence of ATCC strain PTA-126787, or a variant thereof comprising or having a nucleic acid sequence at least 98%, at least 98.5%, at least 99%, or at least 99.5% sequence identity to the genomic nucleic acid sequence of ATCC strain PTA-126787, or is Lactobacillus reuteri strain 3630, which corresponds to ATCC Patent Deposit Number PTA-126787.