Production method for product

Abstract

An object of the present invention is to provide a method in which a proliferation capacity of cells is maintained and a product having high quality is obtained with high productivity over a long period of time, in a production method for a product by cell culture. According to the present invention, there is provided A production method for a product, including: performing perfusion culture of cells in a culture solution, in which a perfusion ratio P (vvd) in the perfusion culture, a cell density V (×106 cells/ml) in the culture solution, and X, which is a ratio of an expression level of an isovaleryl-CoA dehydrogenase gene Ivd to an expression level of a glycerol-3-phosphate dehydrogenase gene Gpdh in the culture solution, satisfy 1.0×10−5≤X×(P/V)≤1.0×10−4.

Dependencies are inferred from the claim text.