Isolation kit

A portable and combined isolation kit that will enable obtaining activated exosome molecules, nanoparticles or protein molecules in certain sizes from various sources including cell culture, biological material, plant, blood plasma, wherein the isolation kit comprises the following:

• an activation chamber with glass beads inside so as to enable an activation of exosomes, nanoparticles or protein molecules,
• a filter element so as to filter molecules larger than the desired molecular size in an activated material,
• a filtration chamber with a tangential-flow filtering element inside so as to increase a filtration and concentration of undesirably small molecules and liquid in the filtered material with large molecules,
• an inlet port number 1 so as to connect a syringe containing a material to be activated to the isolation kit,
• an inlet port number 2 wherein the inlet port number 2 is connected to a syringe which the material will fill when pressed from the syringe connected to the inlet port number 1 and passes through the activation chamber with the glass beads,
• an inlet port number 3 to wherein the inlet port number 3 is connected to the syringe connected to the inlet port number 2, which is filled with activated material inside after 15-20 transfers between the syringe connected to the inlet port number 1 and the syringe connected to the inlet port number 2, is connected for filtering, after being removed from the inlet port number 2,
• an inlet port number 4, wherein the inlet port number 4 is connected to a syringe which the molecules larger than desired and in the material pressed from the syringe that is removed from the inlet port number 2 and connected to the inlet port number 3 will be filled after being filtered by the filter element,
• an inlet port number 5, where the syringe in the inlet port number 4 will be removed and attached, so as to filter the molecules found in the material activated by repeated passing through glass beads, and whose molecules larger than desired are filtered by passing though the filter element and to filter out undesirably small molecules,
• an inlet port number 6 so as to connect a syringe into which molecules of desired sizes will be filled while the material pressed from the syringe attached to the inlet port number 5 after being removed from the inlet port number 4, passes through the tangential flow filtering element in the filtration tube,
• an inlet port number 7 so as to connect a syringe into which molecules and liquid of smaller sizes than desired will be filled while the material pressed from the syringe attached to the inlet port number 5, passes through the tangential flow filtering element in the filtration tube.