Systems, Methods And Compositions For The Preservation And Rehabilitation Of Living Allogenic Heart Valves

A culture vessel configured for culturing a biological tissue, the culture vessel comprising:

• a chamber configured to receive a biological tissue;
• and
• a conduit in communication with the chamber and configured to facilitate a circumferential flow of a composition,
• wherein at least a portion of the chamber and the conduit are configured to be mounted to a support, and
• wherein the chamber and the conduit are configured to be rotated about an axis of rotation so as to cause the composition to flow through the chamber,
• optionally wherein at least a portion of the culture vessel is substantially annular.

A method for culturing biological tissue, the method comprising:

• rotating an annular culture vessel having a biological tissue retained therein so as to give rise to motion of a fluid composition within the annular culture vessel,
• the biological tissue optionally secured within a chamber of the annular culture vessel;
• and
• the rotating giving rise to flow of the fluid composition along the biological tissue,
• the flow of the fluid composition optionally recapitulating fluid flow conditions experienced by the biological tissue in vivo.

A composition for tissue preservation, comprising:

• a base solvent;
• at least one of an animal serum and an animal-free serum;
• an antioxidant;
• insulin, an insulin analog, or an insulin mimetic;
• a glutamine;
• at least one of an antibiotic and an antimycotic;
• and
• optionally at least one of albumin and ascorbic acid.